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Sequencing of surface immobilized polymers utilizing microflourescence detection

  • US 7,329,496 B2
  • Filed: 01/05/2006
  • Issued: 02/12/2008
  • Est. Priority Date: 12/06/1990
  • Status: Expired due to Fees
First Claim
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1. A method for determining the incorporation of nucleotides comprising:

  • (a) providing a plurality of target template nucleic acids attached to a substrate in positionally separated regions at a density of at least 1000 regions per cm2;

    (b) hybridizing at least one primer to said plurality of target template nucleic acids to form hybridized primer;

    (c) contacting said hybridized primer with, at least one nucleotide and a template-dependent nucleic acid polymerase, wherein said nucleotide comprises a label, under conditions whereby the hybridized primer is elongated by template-directed addition of a nucleotide; and

    (c) detecting the addition of said nucleotide by detecting the label and thereby determining the incorporation of the nucleotide.

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