System and method for increasing the contrast of an image produced by an epifluorescence microscope
First Claim
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1. A microscope slide for increasing epifluorescent optical contrast of a fluorescently tagged sample emitting an emission light upon illumination by an excitation light, comprising:
- a top surface; and
a bottom surface;
a sample-proximal infrared reflecting film deposited on said top surface, wherein the infrared reflecting film at least partially reflects an infrared focusing light beam at or near a sample focal point or focal plane; and
a dichroic reflecting film deposited on either said top surface, adjacent to, or in contact with, the sample, or said bottom surface, underneath the sample, wherein said dichroic reflecting film reflects the emission light emitted by said sample while transmitting the excitation light illuminating said sample.
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Abstract
The contrast of an image produced by epifluorescence microscopy may be increased by placing a high-pass dichroic reflecting film behind the sample. The reflecting film reflects the emission light emitted by the fluorescent tags in the sample back through the objective lens while allowing the shorter wavelength excitation light to pass through the sample holder.
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Citations
13 Claims
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1. A microscope slide for increasing epifluorescent optical contrast of a fluorescently tagged sample emitting an emission light upon illumination by an excitation light, comprising:
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a top surface; and a bottom surface; a sample-proximal infrared reflecting film deposited on said top surface, wherein the infrared reflecting film at least partially reflects an infrared focusing light beam at or near a sample focal point or focal plane; and a dichroic reflecting film deposited on either said top surface, adjacent to, or in contact with, the sample, or said bottom surface, underneath the sample, wherein said dichroic reflecting film reflects the emission light emitted by said sample while transmitting the excitation light illuminating said sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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Specification