Methods and reagents for the rapid and efficient isolation of circulating cancer cells
First Claim
1. A coated magnetic particle comprising a nanoparticle core material of magnetite, a protein base coating material of bovine serum albumin directly coated onto quasi-stable crystalline clusters of said core material and an additional streptavidin coating coupled to said base coating material, wherein a fraction of the streptavidin which is coupled to said base coating material is bound to biotinylated antibody and substantial portion of said streptavidin remaining is bound to biotin-bovine serum albumin conjugate.
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Abstract
A highly sensitive assay is disclosed which combines immunomagnetic enrichment with multiparameter flow cytometric and immunocytochemical analysis to detect, enumerate and characterize carcinoma cells in the blood. The assay can detect one epithelial cell or less in 1 ml of blood and has a greater sensitivity than conventional PCR or immunohistochemistry by 1-2 orders of magnitude. In addition, the assay facilitates the biological characterization and staging of carcinoma cells.
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Citations
6 Claims
- 1. A coated magnetic particle comprising a nanoparticle core material of magnetite, a protein base coating material of bovine serum albumin directly coated onto quasi-stable crystalline clusters of said core material and an additional streptavidin coating coupled to said base coating material, wherein a fraction of the streptavidin which is coupled to said base coating material is bound to biotinylated antibody and substantial portion of said streptavidin remaining is bound to biotin-bovine serum albumin conjugate.
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3. A coated, magnetic particle within a population of coated, magnetic nanoparticles, said coated, magnetic particle comprising:
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a. a fractionated nanoparticle core between 90 nm and 150 nm that provides between 83% and 93% target recovery in external magnetic gradient wherein said core comprises at least one transition metal oxide; b. a base coating material adsorbed onto said nanoparticle core wherein said material comprises a protein directly coated onto a quasi-stable crystalline cluster of said fractionated nanoparticle core; and c. an additional streptavidin coating coupled to said base coating material, wherein a fraction of the streptavidin which is coupled to said base coating material is bound to biotinylated antibody and substantial portion of said streptavidin remaining is bound to biotin-bovine serum albumin conjugate.
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- 4. A coated magnetic particle comprising a nanoparticle core material having a magnetic transition metal oxide core, a protein base coating material coated on the nanoparticle core and an additional streptavidin coating coupled to said base coating material through a bifunctional linking compound, wherein a fraction of the streptavidin which is coupled to said base coating material is bound to biotinylated antibody and substantial portion of said streptavidin remaining is bound to biotin-bovine serum albumin conjugate.
Specification