Method for detecting analyte(s) using magnetic colloidal particles

• US 7,332,353 B2
• Filed: 11/20/2002
• Issued: 02/19/2008
• Est. Priority Date: 11/20/2001
• Status: Expired due to Fees

• Current Assignee: Bibette Jerome, Diagnostica Stago (Stago International)
• Original Assignee: Jerome Bibette, Diagnostica Stago (Stago International)
• Inventors: Bibette, Jérôme, Rousseau, Alain, Baudry, Jean
• Primary Examiner(s): Lam; Ann Y.
• Application Number:
  US10/496,114
• Publication Number:
  US 20050048673A1
• Time in Patent Office: 1,917 Days
• Field of Search: 436/526, 436/514, 436/517, 436/518, 436/523, 436/524, 436/525, 436/527, 436/172, 436/149, 435/4, 435/7.1, 435 791- 795, 4352871-2873, 422/73
• US Class Current: 436/526
• CPC Class Codes: G01N 33/5434 using magnetic particle immunoreagent carriers which constitute new materials per se

Abstract

The invention concerns a method for detecting and/or quantifying at least an analyte in a liquid medium, characterized in that it consists in using magnetic colloidal particles functionalized at the surface with at least a ligand specific of the analyte to be detected and/or assayed and in that it comprises: contacting said particles with said medium to be analyzed; applying a magnetic field to said medium, at an intensity sufficient to cause said magnetic particles to be structured in the form of chains; maintaining said magnetic field for a sufficient time interval to enable coupling or combination of the analyte concerned with at least two specific ligands present respectively on two neighbouring particles of a chain; cancelling the magnetic field, and determining the presence and/or absence of said analyte and, as the case may be, its concentration via the presence and/or the absence of said permanent chains of magnetic particles.

Claims

1. A method for detecting or quantifying at least one analyte in a liquid medium, said method comprising:

providing magnetic colloidal particles functionalized at the surface with at least one ligand specific for an analyte to be detected or assayed;

pretreating the medium to be analyzed beforehand in order to make the analyte being sought reactive with respect to at least two molecules of one of its specific ligands, wherein the pretreatment comprises functionalizing the analyte with one of the partners of a high affinity binding couple;

bringing said particles into contact with said medium to be analyzed;

applying a continuous magnetic field to said medium, at a strength sufficient to cause said magnetic particles to be structured in the form of chains;

maintaining said continuous magnetic field for a period of time sufficient to enable coupling or combination of at least one of the analytes concerned with at least two specific ligands present respectively on two consecutive neighboring particles of a chain;

removing the magnetic field after the period of time sufficient to enable coupling or combination of at least one of the analytes; and

determining the presence or absence of at least one of the analytes after removing the magnetic field and, where appropriate, a respective concentration of at least one analyte by the presence or absence of permanent chain(s) of magnetic colloidal particles.

2. The method as claimed in claim 1, wherein said particles are superparamagnetic colloidal particles.

3. The method as claimed in claim 2, wherein the superparamagnetic colloidal particles are obtained by coprecipitation of a polymer with an aqueous ferrofluid or by emulsification with a ferrofluid in organic phase.

4. The method as claimed in claim 1, wherein the magnetic colloidal particles are between 5 and 10,000 nm in size.

5. The method as claimed in claim 1, wherein the specific ligands are immobilized at the surface of the particles by absorption interactions, covalent interactions and/or high affinity interactions.

6. The method as claimed in claim 1, wherein the immobilized ligand is one of the two partners of a high affinity binding couple.

7. The method as claimed in claim 6, wherein the ligand is chosen from one of the partners of the couples (poly)carbohydrates/electin, biotin or biotinylated compounds/avidin or streptavidin, protein receptor and its specific ligand, and haptene/antibody.

8. The method as claimed in claim 1, wherein the immobilized ligand is a compound chosen from peptides, proteins, including glycoproteins, lipoproteins, in free or complex form, immunoglobins, nucleic acids, saccharides, lipids, hormones, receptors, metabolites or other biological substances.

9. The method as claimed in claim 1, wherein the analytes targeted are antigens, antibodies and nucleic acids and/or proteins.

10. The method as claimed in claim 1, for detecting and/or quantifying (an) analyte(s) of the type antibodies to pathogens, antibodies against tumors, antibodies directed against allergens, or autoantibodies.

11. The method as claimed in claim 1, wherein the at least one analyte is an antigen.

12. The method as claimed in claim 1, wherein the magnetic colloidal particles also carry a secondary label.

13. The method as claimed in claim 12, wherein the secondary label can be characterized visually, optically, mechanically and/or electrically.

14. The method as claimed in claim 12, wherein the secondary label is chosen from color pigments and fluorescence agents or phosphore-scence agents.

15. The method as claimed in claim 1, wherein several populations of magnetic colloidal particles functionalized, respectively, with at least one ligand specific for the various analytes to be detected are used.

16. The method as claimed in claim 1, wherein the magnetic field is continuous.

17. The method as claimed in claim 16, wherein the continuous magnetic field is from 5 to 500 mT.

18. The method as claimed in claim 1, wherein the characterization of the chains is carried out directly by visualization under a microscope and/or indirectly by any optical, mechanical or electrical method.

19. The method as claimed in claim 18, wherein the characterization of the chains is carried out by photometry or turbidimetry.

20. The method as claimed in claim 18, wherein the characterization of the chains is carried out by image processing.

21. The method as claimed in claim 20, wherein a CCD camera is used as image-capturing device.

22. The method as claimed in claim 18, wherein a laser is used as light source.

23. The method as claimed in claim 1, wherein the liquid medium is in a microfluid system.

24. The method of claim 1, wherein the magnetic colloidal particles are between 100 and 500 nm in size.