Immersion sensor for measuring the concentration of an analyte with the help of an oxidase
First Claim
1. An immersion sensor for measuring the concentration of at least one analyte with the aid of an oxidase, wherein said immersion sensor comprises:
- a base body wherein the outside surface of said base body is defined by an analyte-permeable region and an analyte-impermeable, oxygen-permeable region which is a membrane,an enzyme region comprising an oxidase within said base body and connected to said membrane,a pair of electrodes within said base body and adjacent to said enzyme region, andat least one water-containing channel within said base body which connects the enzyme region to the analyte-permeable region of the outside surface of said base body.
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Abstract
The invention relates to measuring the concentration of analytes with the aid of an oxidase in an immersion sensor situated in a fluid or in a matrix containing fluid. Oxygen diffuses into the enzyme layer from within, from a gas-filled space connected to the atmosphere and/or to an oxygen reservoir. This enables oxygen saturation of the oxidase in a low-oxygen or oxygen-free medium and/or at high analyte concentrations. The analyte diffuses into the enzyme layer in a channel or a number of channels which contain(s) water and limit(s) diffusion, wherein the channel/channels on the surface of the sensor is/are filled with a protein-impermeable, hydrophilic matrix. By increasing the channel cross-section on the surface of the sensor and/or by connecting the channel/channels to a protein-impermeable, porous, hydrophilic layer on the surface of the sensor, the effect of outer deposits on the diffusion resistance of the analyte is reduced.
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Citations
20 Claims
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1. An immersion sensor for measuring the concentration of at least one analyte with the aid of an oxidase, wherein said immersion sensor comprises:
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a base body wherein the outside surface of said base body is defined by an analyte-permeable region and an analyte-impermeable, oxygen-permeable region which is a membrane, an enzyme region comprising an oxidase within said base body and connected to said membrane, a pair of electrodes within said base body and adjacent to said enzyme region, and at least one water-containing channel within said base body which connects the enzyme region to the analyte-permeable region of the outside surface of said base body. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. An immersion sensor for measuring the concentration of at least one analyte with the aid of an oxidase, wherein said immersion sensor comprises:
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a base body wherein the outside surface of said base body is defined by an analyte-permeable region and an analyte-impermeable, oxygen-permeable region which is a substantially continuous membrane, an enzyme region comprising an oxidase within said base body and connected to said substantially continuous membrane, a pair of electrodes within said base body and adjacent to said enzyme region; and at least one water-containing channel within said base body which connects the enzyme region to the analyte-permeable region of the outside surface of said base body. - View Dependent Claims (20)
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Specification