Method and apparatus for automatic pin detection in microarray spotting instruments

• US 7,335,338 B2
• Filed: 04/11/2005
• Issued: 02/26/2008
• Est. Priority Date: 03/20/2000
• Status: Expired due to Fees

• Current Assignee: PerkinElmer LAS, Inc. (Perkinelmer Incorporated)
• Original Assignee: PerkinElmer LAS, Inc. (Perkinelmer Incorporated)
• Inventors: Candiloro, C. Brian, Schermer, Mack J.
• Primary Examiner(s): Warden; Jill
• Assistant Examiner(s): Handy; Dwayne K
• Application Number:
  US11/103,162
• Publication Number:
  US 20050181510A1
• Time in Patent Office: 1,051 Days
• Field of Search: 422/62, 422/67, 422/99, 422/100, 436/43, 436/49, 436/54, 436/180, 738/63, 738/630.1, 738/64, 738/640.1, 738/642.3, 738/633.1, 738/633.2
• US Class Current: 422/521
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00689 using computers
• CPC Class Codes: B01J 2219/00693 Means for quality control
• CPC Class Codes: B01L 3/0241 Drop counters; Drop formers making arrays for combinatorial libraries B01J19/0046; automation of dispensing for analysis G01N35/10
• CPC Class Codes: B01L 3/0244 using pins
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/114998 with treatment or replacement of aspirator element [e.g., cleaning, etc.]
• CPC Class Codes: Y10T 436/119163 : with aspirator of claimed structure
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

A method and apparatus are provided for automatically sensing the presence (or absence) of spot dispensers such as pins in various possible mounting locations in the printhead of a microarray spotting instrument. Pin-location data obtained by the method and apparatus is provided to the computer controller of the instrument, which uses the data to control the motion of the printhead during operation of the instrument. A pin detection apparatus includes one or more sensor elements that automatically sense possible pin locations in the printhead for the presence of pins. The sensor elements are preferably arranged in an array corresponding to the array of pin locations in the printhead so that pin detection at each pin location can be performed simultaneously. Various types of sensor elements can be used to locate pins in a printhead. For example, sensors can be used that are remote from or attached to a printhead. Pin sensors can be used that sense pin locations from various positions relative to the printhead including above or below the printhead. Also, pin sensing can be by contact or non-contact mechanisms. The method and apparatus allow pin positions in a printhead to be quickly and accurately determined, and without significant risk of damaging the pins, which are fragile.

Claims

1. A method of determining the arrangement of fluid dispensing pins in a printhead of a microarray spotting instrument, said printhead having a plurality of pin locations, at least some of which each include a fluid dispensing pin disposed therein, the method comprising:

automatically detecting whether a fluid dispensing pin is present at each said pin location to determine the arrangement of fluid dispensing pins in said printhead; and

generating a signal indicating said arrangement of fluid dispensing pins in said printhead.

2. The method of claim 1 wherein automatically detecting whether a fluid dispensing pin is present at each said pin location comprises using a sensor to sense whether a fluid dispensing pin is present in a each pin location.

3. The method of claim 2 wherein said sensor comprises a contact and circuitry for detecting electrical conduction between said contact and said fluid dispensing pin.

4. The method of claim 3 wherein said contact comprises a probe hole in a printed circuit board.

5. The method of claim 3 wherein said contact is compliant.

6. The method of claim 5 wherein said contact is spring-mounted.

7. The method of claim 5 wherein said contact comprises a conductive rubber pad.

8. The method of claim 2 wherein said sensor senses the presence of a pinhead of the fluid dispensing pin.

9. The method of claim 2 wherein said sensor senses the presence of a tip of the fluid dispensing pin.

10. The method of claim 2 wherein said sensor is a strain gage sensor, a piezoelectric sensor, a micro-switch, a capacitive sensor, an optical sensor, or an inductive sensor.

11. The method of claim 2 wherein said sensor is an inductive sensor that includes an air-core coil inductor with a hole for receiving at least a portion of a pin.

12. The method of claim 2 wherein said sensor is an inductive sensor that comprises a pair of spaced apart, but proximate coils each adapted for receiving a portion of a fluid dispensing pin.

13. The method of claim 1 wherein automatically detecting whether a fluid dispensing pin is present at each said pin location comprises using a pin detection apparatus comprising a plate with a hole by applying a vacuum to said hole;

positioning said printhead relative to said plate such that if a fluid dispensing pin were located in a given pin location of said printhead, said fluid dispensing pin would be inserted in said hole; and

sensing any pressure changes in said hole.

14. The method of claim 1 wherein automatically detecting whether a fluid dispensing pin is present at each said pin location comprises using a pin detection apparatus comprising a surface with a step, and wherein using said apparatus comprises:

positioning said printhead relative to said step such that a given pin location is positioned above said step and if a fluid dispensing pin were located in said given pin location, said fluid dispensing pin would be supported by said step and a pinhead of said fluid dispensing pin would be raised relative to the printhead; and

sensing any pinhead raised relative to said printhead.

15. The method of claim 1 wherein automatically detecting whether a fluid dispensing pin is present at each said pin location comprises using a pin detection apparatus having an array of sensor elements corresponding in arrangement to said pin locations in said printhead for simultaneously detecting the presence of a fluid dispensing pin in each pin location.

16. The method of claim 1 wherein said fluid dispensing pins comprise solid pins, split pins, or tubular pins.

17. The method of claim 1 further comprising automatically detecting whether any of said fluid dispensing pins are stuck in a raised position in the printhead.

18. An apparatus used in a microarray spotting instrument for determining which pin locations in a printhead of the microarray spotting instrument are occupied by fluid dispensing pins, comprising at least one sensor element to automatically detect whether a fluid dispensing pin is present in each said pin location for determining the arrangement of a plurality of fluid dispensing pins in said printhead.

19. The apparatus of claim 18 wherein said at least one sensor element comprises a contact and circuitry for detecting electrical conduction between said contact and said fluid dispensing pin.

20. The apparatus of claim 19 wherein said contact comprises a probe hole in a printed circuit board.

21. The apparatus of claim 19 wherein said contact is compliant.

22. The apparatus of claim 19 wherein said contact is spring-mounted.

23. The apparatus of claim 19 wherein said contact comprises a conductive rubber pad.

24. The apparatus of claim 18 wherein said at least one sensor element senses the presence of the pinhead of the fluid dispensing pin.

25. The apparatus of claim 18 wherein said at least one sensor element senses the presence of a tip of the fluid dispensing pin.

26. The apparatus of claim 18 wherein said at least one sensor element comprises a strain gage sensor, a piezoelectric sensor element, a micro-switch, a capacitive sensor, an optical sensor or an inductive sensor.

27. The apparatus of claim 18 wherein said at least one sensor element comprises an inductive sensor having an air-core coil inductor including a hole for receiving at least a portion of a fluid dispensing pin.

28. The apparatus of claim 18 wherein said at least one sensor element comprises an inductive sensor comprising a pair of spaced apart, but proximate coils each adapted for receiving a portion of a fluid dispensing pin.

29. The apparatus of claim 18 wherein said at least one sensor element comprises a vacuum sensor including a plate with a hole, means for applying a vacuum to said hole, and a pressure transducer at said hole for sensing pressure changes at said hole from placing a fluid dispensing pin in said hole.

30. The apparatus of claim 18 wherein said at least one sensor element comprises a surface with a step and a sensor for detecting a raised pinhead of a fluid dispensing pin in the printhead when the printhead is positioned relative to said step such that said fluid dispensing pin is supported by said step.

31. The apparatus of claim 18 wherein said at least one sensor element comprises an array of sensor elements corresponding in arrangement to said fluid dispensing pin locations in said printhead for simultaneously detecting the absence or presence of fluid dispensing pins in each pin location.

32. The apparatus of claim 18 wherein said pins comprise solid pins, split pins, or tubular pins.

33. The apparatus of claim 18 wherein said at least one sensor element also automatically detects whether a fluid dispensing pin is stuck in a raised position in the printhead.

34. A microarray spotting instrument comprising:

a printhead having a plurality of pin locations;

a plurality of fluid dispensing pins movably or releasably mounted in at least a subset of the pin locations of the printhead;

a substrate station for holding at least one microarray substrate;

a well station for holding sample material to be deposited on said at least one microarray substrate using said printhead;

a positioning mechanism for moving said printhead;

a controller for controlling movement of said printhead; and

a pin detection apparatus comprising at least one sensor element to automatically detect whether a fluid dispensing pin is present in each pin location for automatically determining the arrangement of fluid dispensing pins in said printhead.

35. In a microarray spotting instrument, a method of providing to a controller operating the instrument information on the arrangement of fluid dispensing pins in a printhead in the instrument, comprising:

providing a printhead having a plurality of pin locations at which fluid dispensing pins are movably or releasably positionable;

automatically sensing whether a pin is present in each pin location to determine the arrangement of pins in the printhead and generating a signal indicative thereof; and

transmitting the signal to the controller.