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Glyphosate tolerant sugar beet

  • US 7,335,816 B2
  • Filed: 02/28/2003
  • Issued: 02/26/2008
  • Est. Priority Date: 02/28/2003
  • Status: Expired due to Term
First Claim
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1. An event H7-1 sugar beet plant comprising a transgenic insert that comprises a transgene which confers glyphosate tolerance, wherein the event H7-1 plant can be identified by using a pair of primers, at least one of which hybridizes to genomic DNA outside of the insert, and wherein the pair of primers can be used in polymerase chain reaction to amplify from the DNA of said sugar beet plant, cells, tissues or seeds thereof, a DNA fragment comprising genomic DNA and DNA of at least part of the insert, wherein the amplified fragment and pair of primers are selected from the group consisting of:

  • (a) a DNA fragment of 630-700 bp that can be amplified using a first primer having the nucleotide sequence of SEQ ID NO;

    1 and a second primer having the nucleotide sequence of SEQ ID NO;

    2;

    (b) a DNA fragment of 3500-3900 bp that can be amplified using a first primer having the nucleotide sequence of SEQ ID NO;

    3 and a second primer having the nucleotide sequence of SEQ ID NO;

    4;

    (c) a DNA fragment of 270-300 bp that can be amplified using a first primer having the nucleotide sequence of SEQ ID NO;

    7 and a second primer having the nucleotide sequence of SEQ ID NO;

    8;

    (d) a DNA fragment of 710-790 bp that can be amplified a first primer having the nucleotide sequence of SEQ ID NO;

    9 and a second primer having the nucleotide sequence of SEQ ID NO;

    10;

    (e) a DNA fragment of 990-1100 bp that can be amplified a first primer having the nucleotide sequence of SEQ ID NO;

    14 and a second primer having the nucleotide sequence of SEQ ID NO;

    16; and

    (f) any combination of elements (a) through (e).

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