Glyphosate tolerant sugar beet

• US 7,335,816 B2
• Filed: 02/28/2003
• Issued: 02/26/2008
• Est. Priority Date: 02/28/2003
• Status: Expired due to Term

• Current Assignee: KWS SAAT SE
• Original Assignee: KWS SAAT AG
• Inventors: Kraus, Josef, Sauerbrey, Elke, Loock, Andreas, Nehls, Reinhard, Jansen, Rudolf
• Primary Examiner(s): Kruse; David H
• Application Number:
  US10/376,763
• Publication Number:
  US 20040172669A1
• Time in Patent Office: 1,824 Days
• Field of Search: 800/300, 800/267, 800/298
• US Class Current: 800/300
• CPC Class Codes: C12N 15/8275 Glyphosate
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae

Abstract

The invention relates to glyphosate tolerant sugar beet plants, plant material and seeds. It is the object of the invention to provide a transgenic sugar beet plant event that shows a high level of tolerance against glyphosate, but is not impaired in other important agronomic properties such as growth, yield, quality, pathogen resistance etc.

Claims

1. An event H7-1 sugar beet plant comprising a transgenic insert that comprises a transgene which confers glyphosate tolerance, wherein the event H7-1 plant can be identified by using a pair of primers, at least one of which hybridizes to genomic DNA outside of the insert, and wherein the pair of primers can be used in polymerase chain reaction to amplify from the DNA of said sugar beet plant, cells, tissues or seeds thereof, a DNA fragment comprising genomic DNA and DNA of at least part of the insert, wherein the amplified fragment and pair of primers are selected from the group consisting of:

(a) a DNA fragment of 630-700 bp that can be amplified using a first primer having the nucleotide sequence of SEQ ID NO;

1 and a second primer having the nucleotide sequence of SEQ ID NO;

2;

(b) a DNA fragment of 3500-3900 bp that can be amplified using a first primer having the nucleotide sequence of SEQ ID NO;

3 and a second primer having the nucleotide sequence of SEQ ID NO;

4;

(c) a DNA fragment of 270-300 bp that can be amplified using a first primer having the nucleotide sequence of SEQ ID NO;

7 and a second primer having the nucleotide sequence of SEQ ID NO;

8;

(d) a DNA fragment of 710-790 bp that can be amplified a first primer having the nucleotide sequence of SEQ ID NO;

9 and a second primer having the nucleotide sequence of SEQ ID NO;

10;

(e) a DNA fragment of 990-1100 bp that can be amplified a first primer having the nucleotide sequence of SEQ ID NO;

14 and a second primer having the nucleotide sequence of SEQ ID NO;

16; and

(f) any combination of elements (a) through (e).

2. The event H7-1 sugar beet plant according to claim 1, wherein the DNA fragment of (a) is a 664 bp DNA fragment having the nucleotide sequence of SEQ ID NO:

13.

3. The event H7-1 sugar beet plant according to claim 1, wherein the DNA fragment of (b) is a 3706 bp DNA fragment having the nucleotide sequence of SEQ ID NO:

6.

4. The event H7-1 sugar beet plant according to claim 1, wherein the DNA fragment of (c) is a 288 bp DNA fragment having the nucleotide sequence of SEQ ID NO:

11.

5. The event H7-1 sugar beet plant according to claim 1, wherein the DNA fragment of (d) is a 751 bp DNA fragment having the nucleotide sequence of SEQ ID NO:

12.

6. The event H7-1 sugar beet plant according to claim 1, wherein the DNA fragment of (e) is a 1042 bp DNA fragment having the nucleotide sequence of SEQ ID NO:

17.

9. The event H7-1 sugar beet plant according to claim 1, wherein the DNA fragment is selected from the group consisting of:

(i) a 664 bp DNA fragment according to (a) having the nucleotide sequence of SEQ ID NO;

13;

(ii) a 3706 bp DNA fragment according to (b) having the nucleotide sequence of SEQ ID NO;

6;

(iii) a 288 bp DNA fragment according to (c) having the nucleotide sequence of SEQ ID NO;

11;

(iv) a 751 bp DNA fragment according to (d) having the nucleotide sequence of SEQ ID NO;

12;

(v) a 1042 bp DNA fragment according to (e) having the nucleotide sequence of SEQ ID NO;

17; and

(vi) any combination thereof.

7. A seed representative of seed deposited with the NCIMB and having the accession number NCIMB 41158 or NCIMB 41159.

8. A transgenic sugar beet plant obtained from seed of claim 7.

10. An event H7-1 sugar beet seed comprising a transgenic insert that comprises a transgene which confers glyphosate tolerance, wherein the event H7-1 seed can be identified by using a pair of primers, at least one of which hybridizes to genomic DNA outside of the insert, and wherein the pair of primers can be used in polymerase chain reaction to amplify from the DNA of said seed a DNA fragment comprising genomic DNA and DNA of at least part of the insert, wherein the amplified fragment and pair of primers are selected from the group consisting of:

(a) a DNA fragment of 630-700 bp that can be amplified using a first primer having the nucleotide sequence of SEQ ID NO;

1 and a second primer having the nucleotide sequence of SEQ ID NO;

2;

(b) a DNA fragment of 3500-3900 bp that can be amplified using a first primer having the nucleotide sequence of SEQ ID NO;

3 and a second primer having the nucleotide sequence of SEQ ID NO;

4;

(c) a DNA fragment of 270-300 bp that can be amplified using a first primer having the nucleotide sequence of SEQ ID NO;

7 and a second primer having the nucleotide sequence of SEQ ID NO;

8;

(d) a DNA fragment of 710-790 bp that can be amplified a first primer having the nucleotide sequence of SEQ ID NO;

9 and a second primer having the nucleotide sequence of SEQ ID NO;

10;

(e) a DNA fragment of 990-1100 bp that can be amplified a first primer having the nucleotide sequence of SEQ ID NO;

14 and a second primer having the nucleotide sequence of SEQ ID NO;

16; and

(f) any combination of elements (a) through (e).

11. The event H7-1 sugar beet seed according to claim 10, wherein the DNA fragment is selected from the group consisting of:

(i) a 664 bp DNA fragment according to (a) having the nucleotide sequence of SEQ ID NO;

13;

(ii) a 3706 bp DNA fragment according to (b) having the nucleotide sequence of SEQ ID NO;

6;

(iii) a 288 bp DNA fragment according to (c) having the nucleotide sequence of SEQ ID NO;

11;

(iv) a 751 bp DNA fragment according to (d) having the nucleotide sequence of SEQ ID NO;

12;

(v) a 1042 bp DNA fragment according to (e) having the nucleotide sequence of SEQ ID NO;

17; and

(vi) any combination thereof.

12. The event H7-1 sugar beet seed according to claim 10, wherein the DNA fragment of (a) is a 664 bp DNA fragment having the nucleotide sequence of SEQ ID NO:

13.

13. The event H7-1 sugar beet seed according to claim 10, wherein the DNA fragment of (b) is a 3706 bp DNA fragment having the nucleotide sequence of SEQ ID NO:

6.

14. The event H7-1 sugar beet seed according to claim 10, wherein the DNA fragment of (c) is a 288 bp DNA fragment having the nucleotide sequence of SEQ ID NO:

11.

15. The event H7-1 sugar beet seed according to claim 10, wherein the DNA fragment of (d) is a 751 bp DNA fragment having the nucleotide sequence of SEQ ID NO:

12.

16. The event H7-1 sugar beet seed according to claim 10, wherein the DNA fragment of (e) is a 1042 bp DNA fragment having the nucleotide sequence of SEQ ID NO:

17.

17. An event H7-1 sugar beet cell, tissue or part, comprising a transgenic insert that comprises a transgene which confers glyphosate tolerance, wherein the event H7-1 cell, tissue or part, can be identified by using a pair of primers, at least one of which hybridizes to genomic DNA outside of the insert, and wherein the pair of primers can be used in polymerase chain reaction to amplify from the DNA of said cell, tissue or part, a DNA fragment comprising genomic DNA and DNA of at least part of the insert, wherein the amplified fragment and pair of primers are selected from the group consisting of:

(a) a DNA fragment of 630-700 bp that can be amplified using a first primer having the nucleotide sequence of SEQ ID NO;

1 and a second primer having the nucleotide sequence of SEQ ID NO;

2;

(b) a DNA fragment of 3500-3900 bp that can be amplified using a first primer having the nucleotide sequence of SEQ ID NO;

3 and a second primer having the nucleotide sequence of SEQ ID NO;

4;

(c) a DNA fragment of 270-300 bp that can be amplified using a first primer having the nucleotide sequence of SEQ ID NO;

7 and a second primer having the nucleotide sequence of SEQ ID NO;

8;

(d) a DNA fragment of 710-790 bp that can be amplified a first primer having the nucleotide sequence of SEQ ID NO;

9 and a second primer having the nucleotide sequence of SEQ ID NO;

10;

(e) a DNA fragment of 990-1100 bp that can be amplified a first primer having the nucleotide sequence of SEQ ID NO;

14 and a second primer having the nucleotide sequence of SEQ ID NO;

16; and

(f) any combination of elements (a) through (e).

18. The event H7-1 sugar beet cell, tissue or part according to claim 17, wherein the DNA fragment is selected from the group consisting of:

(i) a 664 bp DNA fragment according to (a) having the nucleotide sequence of SEQ ID NO;

13;

(ii) a 3706 bp DNA fragment according to (b) having the nucleotide sequence of SEQ ID NO;

6;

(iii) a 288 bp DNA fragment according to (c) having the nucleotide sequence of SEQ ID NO;

11;

(iv) a 751 bp DNA fragment according to (d) having the nucleotide sequence of SEQ ID NO;

12;

(v) a 1042 bp DNA fragment according to (e) having the nucleotide sequence of SEQ ID NO;

17; and

(vi) any combination thereof.

19. The event H7-1 sugar beet cell, tissue or part according to claim 17, wherein the DNA fragment of (a) is a 664 bp DNA fragment having the nucleotide sequence of SEQ ID NO:

13.

20. The event H7-1 sugar beet cell, tissue or part according to claim 17, wherein the DNA fragment of (b) is a 3706 bp DNA fragment having the nucleotide sequence of SEQ ID NO:

6.

21. The event H7-1 sugar beet cell, tissue or part according to claim 17, wherein the DNA fragment of (c) is a 288 bp DNA fragment having the nucleotide sequence of SEQ ID NO:

11.

22. The event H7-1 sugar beet cell, tissue or part according to claim 17, wherein the DNA fragment of (d) is a 751 bp DNA fragment having the nucleotide sequence of SEQ ID NO:

12.

23. The event H7-1 sugar beet cell, tissue or part according to claim 17, wherein the DNA fragment of (e) is a 1042 bp DNA fragment having the nucleotide sequence of SEQ ID NO:

17.

24. An event H7-1 sugar beet plant comprising a transgene that confers glyphosate tolerance, wherein the event H7-1 plant can be identified by using a pair of primers, at least one of which hybridizes to genomic DNA outside of the transgene, and wherein the two primers can be used in polymerase chain reaction to amplify from the DNA of said sugar beet plant, cells, tissues or seeds thereof, a DNA fragment comprising genomic DNA and DNA of at least part of the transgene, wherein the amplified fragment and pair of primers are a DNA fragment of 630-700 bp that can be amplified using a first primer having the nucleotide sequence of SEQ ID NO:

1 and a second primer having the nucleotide sequence of SEQ ID NO;

2.

25. The event H7-1 sugar beet plant according to claim 24, wherein the DNA fragment is a 664 bp DNA fragment having the nucleotide sequence of SEQ ID NO:

13.

26. An event H7-1 sugar beet plant comprising a transgene that confers glyphosate tolerance, wherein the event H7-1 plant can be identified by using a pair of primers, at least one of which hybridizes to genomic DNA outside of the transgene, and wherein the two primers can be used in polymerase chain reaction to amplify from the DNA of said sugar beet plant, cells, tissues or seeds thereof, a DNA fragment comprising genomic DNA and DNA of at least part of the transgene, wherein the amplified fragment and pair of primers are a DNA fragment of 3500-3900 bp that can be amplified using a first primer having the nucleotide sequence of SEQ ID NO:

3 and a second primer having the nucleotide sequence of SEQ ID NO;

4.

27. The event H7-1 sugar beet plant according to claim 26, wherein the DNA fragment is a 3706 bp DNA fragment having the nucleotide sequence of SEQ ID NO:

6.

28. An event H7-1 sugar beet plant comprising a transgene that confers glyphosate tolerance, wherein the event H7-1 plant can be identified by using a pair of primers, at least one of which hybridizes to genomic DNA outside of the transgene, and wherein the two primers can be used in polymerase chain reaction to amplify from the DNA of said sugar beet plant, cells, tissues or seeds thereof, a DNA fragment comprising genomic DNA and DNA of at least part of the transgene, wherein the amplified fragment and pair of primers are a DNA fragment of 990-1100 bp that can be amplified a first primer having the nucleotide sequence of SEQ ID NO:

14 and a second primer having the nucleotide sequence of SEQ ID NO;

16.

29. The event H7-1 sugar beet plant according to claim 28, wherein the DNA fragment is a 1042 bp DNA fragment having the nucleotide sequence of SEQ ID NO:

17.

30. An event H7-1 sugar beet seed comprising a transgene that confers glyphosate tolerance, wherein the event H7-1 seed can be identified by using a pair of primers, at least one of which hybridizes to genomic DNA outside of the transgene, and wherein the two primers can be used in polymerase chain reaction to amplify from the DNA of said sugar beet seed, a DNA fragment comprising genomic DNA and DNA of at least part of the transgene, wherein the amplified fragment and pair of primers are a DNA fragment of 630-700 bp that can be amplified using a first primer having the nucleotide sequence of SEQ ID NO:

1 and a second primer having the nucleotide sequence of SEQ ID NO;

2.

31. The event H7-1 sugar beet seed according to claim 30, wherein the DNA fragment is a 664 bp DNA fragment having the nucleotide sequence of SEQ ID NO:

13.

32. An event H7-1 sugar beet seed comprising a transgene that confers glyphosate tolerance, wherein the event H7-1 seed can be identified by using a pair of primers, at least one of which hybridizes to genomic DNA outside of the transgene, and wherein the two primers can be used in polymerase chain reaction to amplify from the DNA of said sugar beet seed, a DNA fragment comprising genomic DNA and DNA of at least part of the transgene, wherein the amplified fragment and pair of primers are a DNA fragment of 3500-3900 bp that can be amplified using a first primer having the nucleotide sequence of SEQ ID NO:

3 and a second primer having the nucleotide sequence of SEQ ID NO;

4.

33. The event H7-1 sugar beet seed according to claim 32, wherein the DNA fragment is a 3706 bp DNA fragment having the nucleotide sequence of SEQ ID NO:

6.

34. An event H7-1 sugar beet seed comprising a transgene that confers glyphosate tolerance, wherein the event H7-1 seed can be identified by using a pair of primers, at least one of which hybridizes to genomic DNA outside of the transgene, and wherein the two primers can be used in polymerase chain reaction to amplify from the DNA of said sugar beet seed, a DNA fragment comprising genomic DNA and DNA of at least part of the transgene, wherein the amplified fragment and pair of primers are a DNA fragment of 990-1100 bp that can be amplified a first primer having the nucleotide sequence of SEQ ID NO:

14 and a second primer having the nucleotide sequence of SEQ ID NO;

16.

35. The event H7-1 sugar beet seed according to claim 34, wherein the DNA fragment is a 1042 bp DNA fragment having the nucleotide sequence of SEQ ID NO:

17.