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Method for assaying whole blood

  • US 7,338,809 B2
  • Filed: 09/09/2003
  • Issued: 03/04/2008
  • Est. Priority Date: 03/09/2001
  • Status: Expired due to Term
First Claim
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1. A method for measuring quantitatively or qualitatively an analyte in a whole blood sample, comprising:

  • forming a reaction system by mixing the whole blood sample with a whole blood treatment solution comprising detergent of the type that does not cause hemolysis, and adding to the mixture of the whole blood sample and the whole blood treatment solution a first substance which is immobilized on a solid carrier and specifically binds to an analyte contained in the whole blood sample and a second substance which specifically binds to the analyte to allow the analyte to react with the first and second substances to form a complex of first substance-analyte-second substance,separating the complex, anddetecting the complex to measure quantitatively or qualitatively the analyte in the complex,wherein said reaction system comprises the detergent in a concentration range of 0.5 to 5% so that hemolysis is prevented.

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