Integrated circuit chip for bioassays

• US 7,338,811 B2
• Filed: 03/25/2003
• Issued: 03/04/2008
• Est. Priority Date: 03/26/2002
• Status: Expired due to Fees

• Current Assignee: Vincogen Corporation
• Original Assignee: Vincogen Corporation
• Inventors: Lai, Derhsing
• Primary Examiner(s): Le; Long V.
• Assistant Examiner(s): DiRamio; Jacqueline A.
• Application Number:
  US10/396,269
• Publication Number:
  US 20040029109A1
• Time in Patent Office: 1,806 Days
• Field of Search: 422/55, 422/57, 422/61, 422/68.1, 422/82.01, 422/98, 435/4, 435/6, 435/7.1, 435/287.1, 435/287.9, 435/287.2, 436/501, 436/518, 436/524, 436/525, 436/149, 436/150, 436/151
• US Class Current: 436/525
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00567 Transponder chips
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00702 Processes involving means for analysing and characterising the products
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/0074 Biological products
• CPC Class Codes: B01J 2219/00743 Cells
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: G06K 19/0716 at least one of the integrated circuit chips comprising a sensor or an interface to a sensor
• CPC Class Codes: Y02A 90/10 Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation

Abstract

The invention is directed to novel IC chips containing substances used particularly in bioassays. Furthermore, the invention is directed to assay methods and kits using these chips.

Claims

1. An integrated circuit chip for use in an assay of a substance comprising:

a. a non-memory containing transponder having a metal option and read-only tag containing an index ID created by a photomask process, wherein said index ID is transmitted to a receiver in response to a radio signal;

b. at least one substance attached to said transponder, wherein said substance is selected from the group consisting of a biological molecule, hormone, pharmacological agent, tranquilizer, barbiturate, alkaloid, steroid, vitamin, amino acid, sugar, biotin, aviden and streptavidin; and

c. an antenna for receiving and emitting radio signal.

2. The chip according to claim 1, wherein the substance is covalently attached to said transponder.

3. The chip according to claim 1, wherein the substance is noncovalently attached to said transponder.

4. The chip according to claim 1, wherein the substance is a biological molecule.

5. The chip according to claim 4, wherein the biological molecule is selected from the group consisting of a nucleic acid, protein, antibody and at least one cell or subcellular component thereof.

6. The chip according to claim 4, wherein said biological molecule is a member of a phage display library.

7. The chip according to claim 4, wherein said biological molecule is a cell comprising a pathogen.

8. The chip according to claim 1, wherein said substance comprises a detectable moiety.

9. The chip according to claim 1, wherein said transponder comprises binary code.

10. The chip according to claim 1, wherein said transponder further comprises an electrode and said substance is contacted with said electrode.

11. The chip according to claim 1, wherein said substance is contacted with an electrode.

12. The system according to claim 1, wherein said antenna is attached to said transponder.

13. A method for detecting binding of a first substance to a second substance comprising

a. incubating the integrated chip of claim 1, the substance attached to said transponder is a first substance, with a second substance for a time and under conditions for the first and second substances to bind;

b. analyzing the incubated mixture of step a to detect binding of said first substance and said second substance and

c. identifying the substance attached to said transponder.

14. The method according to claim 13, wherein the substance attached to said transponder is identified by decoding information on said transponder.

15. The method according to claim 13, wherein said substance attached to said transponder is a biological sample.

16. The method according to claim 15, wherein said biological sample is selected from the group consisting of a cell, subcellular component, virus and tissue.

17. The method according to claim 13, wherein said substance attached to said transponder is a substance comprising a detectable moiety.

18. The method according to claim 13, wherein said substance attached to said transponder is selected from the group consisting of DNA, RNA, protein, natural product and pharmacological agent.

19. The method according to claim 13, wherein said substance attached to said transponder is an antibody.

20. The method according to claim 19, wherein said antibody is a monoclonal antibody.

21. The method according to claim 13, wherein said substance attached to said transponder is a fusion protein.

22. The method according to claim 13, wherein said substance attached to said transponder is a member of a display library.

23. The method according to claim 13, wherein said substance attached to said transponder is a member of a phage display library.

24. The method according to claim 23, wherein said library is selected from the group consisting of a gene expression library, protein library and antibody library.

25. The method according to claim 17, wherein said substance attached to said transponder is a ligand.

26. The method according to claim 17, wherein said detectable moiety is selected from the group consisting of a chromophore, fluorophore and luminescent agent.

27. The method according to claim 17 wherein said detectable moiety is a luminescent agent selected from the group consisting of a chemiluminescent, photoluminescent, bioluminescent and electrochemiluminescent agent.

28. The method according to claim 13, wherein said transponder further comprises an electrode.

29. The method according to claim 28, wherein said substance is attached to said electrode.

30. The method according to claim 29, wherein said substance attached to said electrode is contacted with a substance having an electrochemiluminescent moiety.

31. The method according to claim 29, wherein said substance attached to said electrode is contacted with a substance containing an electrochemiluminescent moiety.

32. The method according to claim 17, wherein said substance comprising a detectable moiety is selected from the group consisting of an antibody, a protein and a drug.

33. The method according to claim 13, wherein said substance attached to said transponder is a receptor.

34. The method according to claim 13, wherein said substance attached to said transponder is a ligand.

35. The method according to claim 13, wherein said second substance binding to said substance attached to said transponder is a biological sample.

36. The method according to claim 35, wherein said biological sample is selected from the group consisting of a blood, serum, saliva, urine, tissue, and cell sample.

37. The method according to claim 13, wherein said substance attached to said transponder is identified with SPR technology.

38. The method according to claim 13, wherein a detectable moiety is added to the mixture of step (a) before analyzing said mixture.

39. A method for detecting binding of a biological molecule to a substance comprising

a. incubating the integrated chip of claim 1, wherein said substance attached to said transponder on said chip is a biologicaal molecule, with a substance labeled with a detectable moiety;

b. analyzing the incubated mixture of step a to detect binding of said biological molecule to said labeled substance and

c. identifying the biological molecule attached to said transponder.

40. A method for detecting binding of a biological molecule to a substance comprising

a. incubating the integrated circuit chip of claim 1, said substance to said transponder on said chip having a detectable moiety, with a biological molecule;

b. analyzing the incubated mixture of step a to detect binding of said biological molecule to said substance attached to said transponder and

c. identifying the biological molecule attached to said transponder.

41. The method according to claim 39 or claim 40 wherein said biological molecule is a biological sample.

42. The method according to claim 41, wherein said biological sample is selected from the group consisting of tissue, one or more cells, virus particles and subcellular structures.

43. The method according to claim 41, wherein the labeled substance is a pharmacological agent.

44. The method according to claim 41, wherein said detectable moiety is added to the mixture of step a before analyzing said mixture.

45. A method for obtaining the integrated circuit chip of claim 1, comprising attaching a substance to a transponder containing a tag specific to said transponder, said tag containing information which identifies said transponder and is identified by (i) activating said tag with radiowaves and (ii) decoding said information on said tag.

46. The method according to claim 45, wherein the attachment of said biological molecule to said transponder is facilitated with a conjugating agent.

47. The method according to claim 45, wherein said method further comprises contacting said substance with a detectable moiety.

48. A multiplex assay method comprising:

a. providing multiple integrated circuit chips of claim 1 which comprise one or more substances attached to said chips;

b. contacting said chips with one or more substances to determine if said substance binds to a substance on each of said chips;

c. analyzing the incubated mixture of step b to detect binding of said substance to said chips and

d. identifying the substances bound to said substance on said chips.

49. The method according to claim 48, wherein each integrated circuit chip comprises the same substance.

50. The method according to claim 48, wherein said substance on each chip is a biological sample.

51. The method according to claim 48, wherein said substance on one or more chips comprises a detectable moiety.

52. The method according to claim 48, wherein each chip is contacted with more than one substance containing a detectable moiety.

53. The method according to claim 48, wherein each chip comprises the same biological molecule and each chip is contacted with more than one substance.

54. The method according to claim 48 wherein each chip comprises the same substance and each chip is contacted with one or more biological samples.

55. The method according to claim 48, which further comprises adding a detectable moiety to the mixture of step a.

56. The method according to claim 48, which further comprises after step (b) and before step (c) separating the chips from one another.

57. A kit for assaying a biological molecule comprising the integrated circuit chip of claim 1.

58. The kit of claim 57, which further comprises a library of substances.

59. The kit according to claim 58, wherein the library is a phage display library.

60. The kit according to claim 57, which further comprises a detectable moiety.