Ultrasonic analyte concentration and application in flow cytometry
First Claim
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1. An apparatus for concentrating analyte within a fluid using acoustic radiation pressure, said apparatus comprising:
- a function generator to output a radiofrequency electrical signal;
a single acoustic signal producing transducer; and
a tube comprising an inherently axially symmetric geometry;
said single acoustic signal producing transducer acoustically coupled to said tube and used to transform said radiofrequency electrical signal to an acoustic signal;
said tube converting said acoustic signal to said acoustic radiation pressure within said tube, and said acoustic pressure concentrating the analytes within said tube.
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Abstract
The present invention includes an apparatus and corresponding method for concentrating analytes within a fluid flowing through a tube using acoustic radiation pressure. The apparatus includes a function generator that outputs a radio frequency electrical signal to a transducer that transforms the radio frequency electric signal to an acoustic signal and couples the acoustic signal to the tube. The acoustic signal is converted within the tube to acoustic pressure that concentrates the analytes within the fluid.
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Citations
26 Claims
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1. An apparatus for concentrating analyte within a fluid using acoustic radiation pressure, said apparatus comprising:
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a function generator to output a radiofrequency electrical signal; a single acoustic signal producing transducer; and a tube comprising an inherently axially symmetric geometry; said single acoustic signal producing transducer acoustically coupled to said tube and used to transform said radiofrequency electrical signal to an acoustic signal; said tube converting said acoustic signal to said acoustic radiation pressure within said tube, and said acoustic pressure concentrating the analytes within said tube. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method for concentrating analyte within a fluid using acoustic radiation pressure, the method comprising:
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producing the acoustic radiation pressure in a tube comprising an inherently axially symmetric geometry using a single acoustic signal producing transducer; flowing a fluid with the analyte through the tube; driving the transducer and inducing an outer boundary surface displacement; and concentrating the analyte within the tube. - View Dependent Claims (9)
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10. A flow cytometer for the analysis of analytes, comprising:
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a tube having an inlet for accepting a fluid sample stream of the analytes; an acoustic signal producing transducer coupled to said tube, wherein said acoustic signal producing transducer produces an acoustic signal to said tube to induce within said tube acoustic radiation pressure capable inducing an outer boundary surface displacement to concentrate the analytes within the fluid sample stream; optical equipment for analyzing the analytes wherein said optical equipment comprises a light source to create light scatter along with several wavelength bands of fluorescence for analyzing the analytes; and collection optics to receive the light scatter and the several wavelength bands of fluorescence created to determine molecular makeup of the analytes. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18)
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19. A method of flow cytometry, comprising:
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supplying a fluid stream with analytes to an inlet of a tube; subjecting the tube to acoustic radiation pressure to induce an outer boundary surface displacement to concentrate the analytes within the fluid stream; and analyzing the analytes via a flow cytometer. - View Dependent Claims (20)
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21. A method of flow cytometry comprising:
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supplying a fluid stream with analytes to an inlet of a tube; subjecting the tube to acoustic radiation pressure to induce an outer boundary surface displacement to concentrate the analytes within the fluid stream; and analyzing the analytes with optical equipment, wherein analyzing the analytes includes subjecting the analytes to a light source that creates light scatter and several bands of fluorescence, and collecting the light scatter and wavelength bands of fluorescence with an optical detector, and identifying the collected wavelength bands to determine molecular makeup of the analytes. - View Dependent Claims (22)
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23. A flow cytometer for analysis of analytes comprising:
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a tube having an inlet for accepting a fluid stream with analytes; a single acoustic signal producing transducer acoustically coupled to said tube; said single driving transducer producing an acoustic signal that induces acoustic radiation pressure in said tube to produce an outer boundary surface displacement to concentrate the analytes within the fluid stream; and a flow cytometer fluidly connected to said tube for analyzing the analytes. - View Dependent Claims (24, 25, 26)
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Specification