Method and reagent system having a non-regenerative enzyme-coenzyme complex
First Claim
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1. A method for detecting an analyte in a sample by an enzymatic reaction, comprising the steps:
- (a) contacting the sample with a detection reagent comprising an enzyme-coenzyme complex, under conditions with which no regeneration of coenzyme takes place, whereby the enzyme-coenzyme complex is employed in an at least stoichiometric amount relative to the analyte present in the sample, and(b) detecting a reaction of the analyte through a change in the enzyme-coenzyme complex.
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Abstract
The invention relates to a method and reagent system for detecting an analyte in a sample by means of an enzymatic reaction, involving the use of an enzyme-coenzyme complex as a stoichiometric reaction partner for the analyte present in the sample.
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Citations
17 Claims
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1. A method for detecting an analyte in a sample by an enzymatic reaction, comprising the steps:
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(a) contacting the sample with a detection reagent comprising an enzyme-coenzyme complex, under conditions with which no regeneration of coenzyme takes place, whereby the enzyme-coenzyme complex is employed in an at least stoichiometric amount relative to the analyte present in the sample, and (b) detecting a reaction of the analyte through a change in the enzyme-coenzyme complex. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A method for detecting an analyte in a sample by an enzymatic reaction, comprising the steps:
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contacting the sample with a detection reagent comprising a detection reagent comprising an enzyme-coenzyme complex in a form such that no regeneration of the coenzyme takes place, whereby the enzyme-coenzyme complex is employed in an at least stoichiometric amount relative to the analyte present in the sample, and a support to receive the detection reagent, under conditions with which no regeneration of coenzyme takes place, and detecting a reaction of the analyte through a change in the enzyme-coenzyme complex.
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Specification