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Production of motif-specific and context-independent antibodies using peptide libraries as antigens

  • US 7,344,714 B2
  • Filed: 05/07/2002
  • Issued: 03/18/2008
  • Est. Priority Date: 09/04/1998
  • Status: Expired due to Term
First Claim
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1. A method for producing an antibody that specifically binds a single modified amino acid selected from the group consisting of a glycosylated amino acid, an acetylated amino acid, and a methylated amino acid, said method comprising the steps of:

  • (a) constructing a degenerate peptide library comprising (i) a single fixed modified amino acid selected from the group consisting of a glycosylated amino acid, an acetylated amino acid, and a methylated amino acid, and (ii) a plurality of degenerate amino acids flanking said modified amino acid;

    (b) immunizing a host with said peptide library to raise a context-independent antibody specific for said modified amino acid; and

    (c) isolating antisera from said host, and purifying the context-independent antibody of step (b) from said antisera, said antibody specifically binding said single modified amino acid independent of the peptide context in which it occurs.

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