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Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate

  • US 7,344,835 B2
  • Filed: 06/07/2004
  • Issued: 03/18/2008
  • Est. Priority Date: 11/22/1996
  • Status: Expired due to Fees
First Claim
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1. A method of reducing pyrophosphorolysis in a nucleic acid sequencing reaction, said method comprising incubating one or more deoxynucleotides, at least one dideoxynucleotide and a nucleic acid template together with an enzyme selected from the group consisting of a pentosyltransferase, a phosphotransferase with an alcohol group as acceptor, a nucleotidyltransferase, and a carboxy-lyase under conditions sufficient to form a second nucleic acid molecule complementary to all or a portion of said nucleic acid template.

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