Thermal regulation of fluidic samples within a diagnostic cartridge

• US 7,344,894 B2
• Filed: 10/16/2001
• Issued: 03/18/2008
• Est. Priority Date: 10/16/2001
• Status: Expired due to Term

• Current Assignee: Sanofi-Aventis Deutschland GmbH (Sanofi )
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Stawitcke, Frederick, Hartmann, Hans-Joachim, Mauze, Ganapati R., Pering, Richard, Verdonk, Ed, Alden, Don, Soerensen, Olaf, Greenstein, Michael, Ingle, Frank, Drese, Klaus Stefan, Drbal, Vladimir, Pittaro, Rick
• Primary Examiner(s): Lam; Ann Y.
• Application Number:
  US09/981,440
• Publication Number:
  US 20030073229A1
• Time in Patent Office: 2,345 Days
• Field of Search: 436/518, 436/517, 436/8, 436/147, 436/142, 422 50- 55, 422/61, 422/62, 422/105, 422/107, 422/108, 422/109, 422/244, 422284-290, 435/4, 435/5, 435/6, 435/7.1, 435/287.1, 435/287.3, 435/288.4
• US Class Current: 436/518
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2300/1861 using radiation
• CPC Class Codes: B01L 2300/1872 Infrared light
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 7/00 Heating or cooling apparatus autoclaves B01J3/04; Heat insulating devices

Abstract

A method and miniature analytical device with thermal regulation of reactant using a localized heat source capable of emitting electromagnetic radiation, such as light emitting diodes (“LED”s) and vertical cavity surface emitting lasers (“VCSEL”s), generating internal heat, such as resistive, inductive and Peltier heaters, or external heating. The miniature analytical device comprises of array of temperature-controlled zones to restrict the volume heated and localize the heating by having the localized heat source comprise an array of emitters or heaters.

Claims

1. A point of care miniature analytical device with thermal regulation comprising:

a cartridge comprising one or more portions constructed of a material, wherein the one or more portions define an array of temperature-controlled zones including reactants, wherein each said temperature-controlled zones is constrained by cartridge portions that surround an area of space in which a reactant is contained and confine the reactant from flowing into other of said temperature-controlled zones, and wherein the cartridge portions include clear or translucent portions that allow direct irradiation of reactant molecules to facilitate thermal regulation of the reactants and to transmit light through the reactants;

an array of infrared radiation emitting heat sources, wherein the array of heat sources is positioned to correspond to the array of temperature-controlled zones so that each heat source is arranged to provide temperature regulation to a corresponding temperature-controlled zone, and wherein one or more of the heat sources emit localized radiation to provide heating in the corresponding temperature-controlled zone;

an optical temperature monitor, not in contact with the cartridge and disposed adjacent to a portion of the cartridge surrounding the temperature controlled zones, that monitors reactant temperature by measuring electromagnetic radiation;

a controller comprising a modulator;

a power supply configured to supply drive current to the array of heat sources and coupled to the controller to provide that current from the power supply achieves the desired thermal regulation in the temperature-controlled zones;

a feedback loop configured to provide measured temperatures to the controller, and to modulate the power supply to drive the infrared light heat sources to achieve a desired temperature with a smooth control curve at the desired temperature, and

an instrument for measurement of electromagnetic emission obtained from irradiation of the reactants with the infrared radiation emitting heat sources, wherein the transmission of infrared radiation through the reactants allows a determination of a concentration of a material within the reactants.

2. A point of care miniature analytical device with thermal regulation according to claim 1, wherein:

the array of infrared radiation emitting heat sources comprise vertical cavity surface emitting laser light sources.

3. A point of care miniature analytical device with thermal regulation according to claim 1, wherein:

the array of infrared radiation emitting heat sources comprise at least one light source chosen from a vertical cavity surface emitting laser light source, a light emitting diode, an infrared lamp, an infrared laser, and infrared diode laser.

4. A point of care miniature analytical device with thermal regulation according to claim 3, wherein:

at least one of the infrared radiation emitting heat sources in the array of heat sources generates infrared light of a different wavelength from the remainder of the infrared radiation emitting heat sources.

5. A point of care miniature analytical device with thermal regulation according to claim 3, wherein:

the at least one light source generates infrared light with a wavelength of at least 0.775 micrometers.

6. A point of care miniature analytical device with thermal regulation according to claim 3, wherein:

the at least one light source generates infrared light with a wavelength of at most 7000 micrometers.

7. A point of care miniature analytical device with thermal regulation according to claim 1, wherein:

the controller modulates the power supply based on a temperature measured from the zones.

8. A point of care miniature analytical device with thermal regulation according to claim 1, further comprising:

an array of temperature monitors, wherein the array of temperature monitors is positioned to correspond to the array of temperature-controlled zones.

9. A point of care miniature analytical device with thermal regulation according to claim 1, wherein:

the reactants comprise assay elements for body fluid analysis.

10. A point of care miniature analytical device with thermal regulation according to claim 1, wherein:

the array of heat sources provides a reactant temperature that is one or both of achieved with a smooth control curve or maintained at a desired temperature.