Massive parallel method for decoding DNA and RNA
First Claim
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1. A nucleotide analogue which comprises:
- (a) a base which is an adenine, an analogue of adenine, a cytosine, an analogue of cytosine, a guanine, an analogue of guanine, a thymine, an analogue of thymine, a uracil, or an analogue of uracil;
(b) a unique label attached through a cleavable linker to the base or to the analogue of the base;
(c) a deoxyribose;
(d) a —
OR group at a 3′
-position of the deoxyribose, wherein R is —
CH2OCH3 or —
CH2CH═
CH2;
and(e) a triphosphate group.
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Abstract
This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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18 Claims
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1. A nucleotide analogue which comprises:
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(a) a base which is an adenine, an analogue of adenine, a cytosine, an analogue of cytosine, a guanine, an analogue of guanine, a thymine, an analogue of thymine, a uracil, or an analogue of uracil; (b) a unique label attached through a cleavable linker to the base or to the analogue of the base; (c) a deoxyribose; (d) a —
OR group at a 3′
-position of the deoxyribose, wherein R is —
CH2OCH3 or —
CH2CH═
CH2;
and(e) a triphosphate group. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A nucleotide analogue which comprises:
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(a) a base which is an adenine, an analogue of adenine, a cytosine, an analogue of cytosine, a guanine, an analogue of guanine, a thymine, an analogue of thymine, a uracil, or analogue of uracil; (b) a unique label attached through a cleavable linker to the base or to the analogue of the base; (c) a deoxyribose; (d) a —
OR group at a 3′
-position of the deoxyribose, wherein R is a cleavable chemical group; and(e) a triphosphate group, wherein the nucleotide analogue has the structure; - View Dependent Claims (16, 17, 18)
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Specification