Process for separating and deprotecting oligonucleotides
First Claim
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1. A method for deprotecting oligonucleotides, said method comprising:
- a) providing a plurality of oligonucleotides, wherein at least some of said oligonucleotides are each linked to a single trityl or substituted trityl moiety hydrophobic separation function at the 5′
end;
b) precipitating said plurality of oligonucleotides on a hydrophobic solid support by (i) adsorbing said plurality of oligonucleotides onto said hydrophobic solid support, (ii) drying said adsorbate on said hydrophobic solid support, and (iii) contacting said dried adsorbate on said hydrophobic solid support with an organic solvent; and
c) removing said 5′
trityl or substituted trityl moiety hydrophobic separation function from said precipitated oligonucleotides by contact with a non-aqueous solution of acid in order to deprotect said oligonucleotides.
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Abstract
Methods for deprotecting and derivatizing oligonucleotides that are non-covalently bound to a solid support are described. The methods include providing a plurality of oligonucleotides linked to one or more hydrophobic separation functions, wherein the plurality includes protected oligonucleotides, precipitating the plurality of oligonucleotides on a hydrophobic solid support using an organic solvent to produce non-covalently immobilized oligonucleotides, and deprotecting the immobilized oligonucleotides.
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Citations
18 Claims
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1. A method for deprotecting oligonucleotides, said method comprising:
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a) providing a plurality of oligonucleotides, wherein at least some of said oligonucleotides are each linked to a single trityl or substituted trityl moiety hydrophobic separation function at the 5′
end;b) precipitating said plurality of oligonucleotides on a hydrophobic solid support by (i) adsorbing said plurality of oligonucleotides onto said hydrophobic solid support, (ii) drying said adsorbate on said hydrophobic solid support, and (iii) contacting said dried adsorbate on said hydrophobic solid support with an organic solvent; and c) removing said 5′
trityl or substituted trityl moiety hydrophobic separation function from said precipitated oligonucleotides by contact with a non-aqueous solution of acid in order to deprotect said oligonucleotides. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification