Directed microwave chemistry

• US 7,348,182 B2
• Filed: 09/05/2002
• Issued: 03/25/2008
• Est. Priority Date: 10/03/2000
• Status: Expired due to Fees

• Current Assignee: Mirari Biosciences, Inc.
• Original Assignee: Mirari Biosciences, Inc.
• Inventors: Saul, Richard, Martin, Mark T.
• Primary Examiner(s): Le; Long V.
• Assistant Examiner(s): Do; Pensee T.
• Application Number:
  US10/234,092
• Publication Number:
  US 20030082633A1
• Time in Patent Office: 2,028 Days
• Field of Search: 436/518, 436/173, 436/806, 436/350, 436/51, 436/526, 436/149, 435/4, 435/6, 435/7.1, 435/287.1, 435/287.2, 427/2.1, 422/82.01
• US Class Current: 436/518
• CPC Class Codes: B01J 19/126 Microwaves
• CPC Class Codes: B01J 2219/0871 Heating or cooling of the reactor
• CPC Class Codes: B01J 2219/0879 Solid
• CPC Class Codes: C12N 11/00 Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
• CPC Class Codes: G01N 1/44 Sample treatment involving radiation, e.g. heat
• CPC Class Codes: G01N 33/5436 with ligand physically entrapped within the solid phase liposomes G01N33/5432; immunological test elements G01N33/54386
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property
• CPC Class Codes: Y10T 436/115831 Condition or time responsive
• CPC Class Codes: Y10T 436/116664 with automated titrator
• CPC Class Codes: Y10T 436/24 Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Abstract

The present invention concerns a novel means by which chemical preparations can be made. Reactions can be accelerated on special chips using microwave energy. The chips contain materials that efficiently absorb microwave energy causing chemical reaction rate increases. The invention is important in many small scale chemical transformations including those used in protein chemistry and in combinatorial chemistry.

Claims

1. A method for accelerating a biospecific interaction of a reactant in an aqueous or organic solution proximal to a surface, said method comprising:

(a) contacting a composite with said reactant, said composite comprising;

(i) a solid material susceptible to microwave heating attached to (ii) a surface molecule,

(b) applying an electromagnetic field of a wavelength of between 1 cm and 100 m to said composite resulting in direct microwave heating of said solid material, without substantially heating said aqueous or organic solution so that the temperature of the solid material is higher than that of the surface molecule, and

(c) allowing said composite to react with said reactant, wherein the reactant is thermally proximal to the composite, whereby the reactant is heated by heat transfer from the heated solid material and binds to the surface molecule at a greater rate than without heating from the heated soild material, thereby accelerating said biospecific interaction.

2. The method of claim 1, further comprising the step of measuring the extent or rate of said biospecific interaction.

3. The method of claim 1, further comprising the steps of:

(d) contacting said composite with one or more additional reactant(s),

(e) allowing said additional reactant(s) to react in one or more biospecific interactions, and

(f) measuring the extent or rate of one or more of said biospecific interactions.

4. The method of claim 1, further comprising the steps of:

(d) contacting said composite with one or more additional reactant(s) for one or more further biospecific interactions,

(e) applying an electromagnetic field to said composite, said electromagnetic field being sufficient to result in microwave heating of said solid material, said additional reactant(s) being heated by heat transfer from said heated solid material,

(f) allowing said heated additional reactant(s) to react, thereby accelerating one or more of said further biospecific interactions, and

(g) measuring the extent or rate of one or more of said further biospecific interactions.

5. The method of claim 1, further comprising the steps of:

(d) measuring the extent or rate of the reaction,

(e) contacting said composite with one or more additional reactant(s)

(f) applying an electromagnetic field to said composite, said electromagnetic field being sufficient to result in microwave heating of said solid material, said additional reactant(s) being heated by heat transfer from said heated solid material,

(g) allowing one or more of said heated second reactant(s) to react, thereby accelerating one or more of said further biospecific interactions, and

(h) measuring the extent or rate of one or more of said further biospecific interactions.

6. The method of claim 1, wherein said reactant is selected from the group consisting of an amino acid, a protein, a peptide, an oligonucleotide, and a polynucleotide.

7. The method of claim 1, wherein said biospecific interaction is a noncovalent adsorption reaction.

8. The method of claim 1, wherein said reactant is an amino acid.

9. The method of claim 1, where said biospecific interaction is a covalent conjugation reaction.

10. The method of any one of claims 1, 3, 4, or 5 wherein one or more of said biospecific interactions is selected from the group consisting of:

a chemiluminescent reaction, a reaction resulting in a change in visible or ultraviolet color, and a reaction involving a change in fluorescent properties.

11. The method of any one of claims 1, 3, 4, or 5, wherein one or more of said reactant(s) is a product of an enzymatic reaction.

12. The method of claim 1, wherein said solid material susceptible to microwave heating comprises a dielectric material, and wherein said application of said electromagnetic field additionally results in dielectric heating.

13. The method of claim 1, wherein said solution is an aqueous solution and said solid material is more susceptible to microwave heating than water.

14. A method for a biospecific interaction of a biologically active reactant in an aqueous or organic solution proximal to a surface, said method comprising:

(a) contacting a composite with said reactant, said composite comprising;

(i) a solid material susceptible to microwave heating attached to (ii) a surface molecule,

(b) applying an electromagnetic field of a wavelength of between 1 cm and 100 m to said composite resulting in direct microwave heating of said solid material without substantially heating said aqueous or organic solution so that the temperature of the solid material is higher than that of the surface molecule, and

(c) allowing said composite to react with said reactant, wherein the reactant is thermally proximal to the composite so that the heated solid material heats the thermally proximal reactant, whereby the reactant maintains biological activity and binds to the surface molecule at a greater rate than without heating from the heated solid material, thereby accelerating said biospecific interaction while maintaining the biological activity of the reactant.

15. The method of claim 14, wherein said solution is an aqueous solution and said solid material is more susceptible to microwave heating than water.