Integrated active flux microfluidic devices and methods
First Claim
1. A microfluidic device comprising:
- a loop channel communicating with one or more service channels, wherein each service channel comprises a microvalve that when closed separates the service channel and the loop channel and wherein when said microvalves are closed said loop channel is a closed path, anda peristaltic pump comprising at least three cooperating microvalves within the loop channel,wherein the loop channel resides in a layer of elastomeric material and the microvalves comprise an elastomeric membrane.
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Abstract
The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.
221 Citations
63 Claims
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1. A microfluidic device comprising:
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a loop channel communicating with one or more service channels, wherein each service channel comprises a microvalve that when closed separates the service channel and the loop channel and wherein when said microvalves are closed said loop channel is a closed path, and a peristaltic pump comprising at least three cooperating microvalves within the loop channel, wherein the loop channel resides in a layer of elastomeric material and the microvalves comprise an elastomeric membrane. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 46, 47)
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26. A microfluidic device comprising:
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a treatment layer having elastomeric fluid channels comprising a loop channel, a loop inlet channel, and a loop outlet channel, a control layer adjacent to the treatment layer and having elastomeric control channels, wherein at least one control channel intersects each of the inlet and outlet channels to form microvalves, said microvalves comprising an elastomeric membrane between the control channel and the fluid channel which can be deformed where the channels intersect to close the microvalve, and at least three control channels intersect the loop channel to form a peristaltic pump. - View Dependent Claims (27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 48, 49, 50, 51)
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52. A microfluidic device comprising:
- a plurality of loop channels, each loop channel communicating with at least one service channel;
wherein each loop channel is associated with a peristaltic pump comprising at least three microvalves acting within the loop channel. - View Dependent Claims (53, 61, 62, 63)
- a plurality of loop channels, each loop channel communicating with at least one service channel;
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54. A microfluidic device comprising:
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a treatment layer having elastomeric fluid channels comprising at least one inlet channel, at least one outlet channel, and a plurality of loop channels; and a control layer adjacent to the treatment layer and having a plurality of parallel elastomeric control channels, wherein at least three control channels intersect each loop channel to form a peristaltic pump. - View Dependent Claims (55, 56, 57, 58, 59, 60)
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Specification