DNA sequence analysis
First Claim
1. A method for determining the identity of one or more mutations or single nucleotide polymorphisms (SNPs) in a genome, comprising:
- a. contacting a sample genome, under conditions which permit template dependent oligonucleotide ligation, with a plurality of different oligonucleotide molecules which comprise(i) a first set of at least two oligonucleotides, each comprising a sequence of nucleotides that is complementary to a region on said genome that includes a known SNP site, wherein a nucleotide complementary to the known SNP site is at or near the 5′
end of each of said oligonucleotides and, each of said oligonucleotides further comprises a unique label which is a unique coding sequence of nucleotides, wherein said unique coding sequence of nucleotides is specific for the nucleotide complementary to the known SNP site and the position of the SNP to be scored,(ii) a second set of at least two oligonucleotides, each oligonucleotide comprising a sequence of nucleotides complementary to a region on said target genome for hybridisation with said target genome adjacent to the 5′
end of an oligonucleotide of said first set of at least two oligonucleotides, and a surface capture moiety,a phosphate moiety being located at any of either the 5′
end of said first set of at least two oligonucleotides or the 3′
end of said second set of at least two oligonucleotides, wherein said contacting effects hybridization of the first and second set of at least two oligonucleotides to the sample genome and generates ligated oligonucleotides,b. immobilising the ligated oligonucleotides on a solid support via the surface capture moiety to generate immobilised ligated oligonucleotides,c. performing a sequencing reaction on the immobilised ligated oligonucleotides to determine at least the unique coding sequence of nucleotides of one or more of said unique labels, wherein determining the unique coding sequence of nucleotides of a unique label identifies the nucleotide complementary to the known SNP site and the position of the SNP to be scored, and comparing identified nucleotides complementary to known SNP sites in any of said immobilised oligonucleotides to those of one or more reference SNPs.
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Accused Products
Abstract
There is disclosed a method for determining the identity of one or more mutations or single nucleotide polymorphisms (SNPs) in a genome, comprising: a contacting a sample genome, under conditions which permit template dependant oligonucleotide ligation, with a plurality of different oligonucleotide molecules which comprise (i) a first set of oligonucleotides each comprising a sequence of nucleotides that is complementary to a region on said genome that includes a known SNP site and which oligonucleotides are complementary to said region other than at a base at or near the 5′ end of said oligonucleotides that is to be tested for complementarity to a base at the SNP site, each of said oligonucleotides comprising a unique label to identify both the base to be tested and the position of the SNP to be scored, (ii) a second set of oligonucleotides each comprising a sequence of nucleotides complementary to a region on said target genome for hybridisation with said target genome adjacent the 5′ end of an oligonucleotide of said first oligonucleotide set, and a surface capture moiety, a phosphate moiety being located at any of either the 5′ end of said first set of oligonucleotides or the 3′ end of said second set of oligonucleotides, any resulting ligated oligonucleotide being immobilised on a solid support via the surface capture moiety, b. analysing said solid support for the identity of one or more of said unique labels and comparing the defined bases in any of said immobilised oligonucleotides to those of the reference one or more SNPs.
9 Citations
11 Claims
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1. A method for determining the identity of one or more mutations or single nucleotide polymorphisms (SNPs) in a genome, comprising:
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a. contacting a sample genome, under conditions which permit template dependent oligonucleotide ligation, with a plurality of different oligonucleotide molecules which comprise (i) a first set of at least two oligonucleotides, each comprising a sequence of nucleotides that is complementary to a region on said genome that includes a known SNP site, wherein a nucleotide complementary to the known SNP site is at or near the 5′
end of each of said oligonucleotides and, each of said oligonucleotides further comprises a unique label which is a unique coding sequence of nucleotides, wherein said unique coding sequence of nucleotides is specific for the nucleotide complementary to the known SNP site and the position of the SNP to be scored,(ii) a second set of at least two oligonucleotides, each oligonucleotide comprising a sequence of nucleotides complementary to a region on said target genome for hybridisation with said target genome adjacent to the 5′
end of an oligonucleotide of said first set of at least two oligonucleotides, and a surface capture moiety,a phosphate moiety being located at any of either the 5′
end of said first set of at least two oligonucleotides or the 3′
end of said second set of at least two oligonucleotides, wherein said contacting effects hybridization of the first and second set of at least two oligonucleotides to the sample genome and generates ligated oligonucleotides,b. immobilising the ligated oligonucleotides on a solid support via the surface capture moiety to generate immobilised ligated oligonucleotides, c. performing a sequencing reaction on the immobilised ligated oligonucleotides to determine at least the unique coding sequence of nucleotides of one or more of said unique labels, wherein determining the unique coding sequence of nucleotides of a unique label identifies the nucleotide complementary to the known SNP site and the position of the SNP to be scored, and comparing identified nucleotides complementary to known SNP sites in any of said immobilised oligonucleotides to those of one or more reference SNPs. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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Specification