BGL3 beta-glucosidase and nucleic acids encoding the same

• US 7,351,573 B2
• Filed: 01/27/2005
• Issued: 04/01/2008
• Est. Priority Date: 09/21/2001
• Status: Expired due to Fees

• Current Assignee: Genencor International Incorporated (International Flavors & Fragrances Incorporated)
• Original Assignee: Genencor International Incorporated (International Flavors & Fragrances Incorporated)
• Inventors: Ward, Michael, Goedegebuur, Frits, Dunn-Coleman, Nigel, Yao, Jian
• Primary Examiner(s): Prouty; Rebecca E.
• Assistant Examiner(s): RAGHU, GANAPATHIRAM
• Application Number:
  US11/046,300
• Publication Number:
  US 20050214921A1
• Time in Patent Office: 1,160 Days
• Field of Search: 435/4, 435/6, 435/69.1, 435/183, 435/200, 435/252.3, 435/320.1, 530/23.27
• US Class Current: 435/254.6
• CPC Class Codes: A21D 8/042 with enzymes
• CPC Class Codes: C11D 3/38645 containing cellulase
• CPC Class Codes: C12N 15/1137 against enzymes viral enzymes C12N15/1131; receptors C12N15/1138
• CPC Class Codes: C12N 9/2445 Beta-glucosidase (3.2.1.21)
• CPC Class Codes: C12Y 302/01021 Beta-glucosidase (3.2.1.21)
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

The present invention provides a novel β-glucosidase nucleic acid sequence, designated bgl3, and the corresponding BGL3 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL3, recombinant BGL3 proteins and methods for producing the same.

Claims

1. A recombinant host cell comprising a deletion or insertion or other alteration in the Trichoderma β

-glucosidase 3 gene which inactivates the gene.

2. A recombinant host cell wherein the recombinant host cell has been modified such that production of a functional Trichoderma β

-glucosidase 3 enzyme is decreased or eliminated by said recombinant host cell wherein said enzyme is encoded by a nucleic acid sequence selected from the group consisting of;

(a) a nucleic acid sequence comprising SEQ ID NO;

1;

(b) a nucleic acid sequence which encodes or is complementary to a sequence which encodes a beta-glucosidase polypeptide having at least 95% sequence identity to the amino acid sequence having SEQ ID NO;

3 and SEQ ID NO;

2;

(c) a nucleic acid sequence which encodes or is complementary to a sequence which encodes a beta-glucosidase polypeptide having the amino acid sequence having SEQ ID NO;

3 and SEQ ID NO;

2;

(d) a nucleic acid sequence which encodes or is complementary to a sequence which encodes a beta-glucosidase polypeptide having the amino acid sequence comprising SEQ ID NO;

2; and

(e) a nucleic acid sequence comDrisinp SEQ ID NO;

4, or the complement thereof, wherein said mutation is deletion or insertion or other alteration to said nucleic acid sequence encoding the said Trichoderma β

-glucosidase 3 enzyme.