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Methods and devices based upon a novel form of nucleic acid duplex on a surface

  • US 7,354,710 B2
  • Filed: 07/11/2002
  • Issued: 04/08/2008
  • Est. Priority Date: 07/11/2001
  • Status: Expired due to Term
First Claim
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1. A biomolecular hybridization device comprising:

  • a substrate having an aminosilanized surface permanently and covalently attached thereto; and

    an adsorbed monolayer formed from an about twice saturating amount of unmodified single-stranded oligonucleotides all of which are about 12 to 16 bases in length adsorbed to the aminosilanized surface as a saturated film of constrained oligonucleotides on the surface via direct non-covalent phosphate-surface adsorptive contact of substantially all phosphate groups of each oligonucleotide at a density of one phosphate group per about 1.5 square nanometers of surface, wherein each constrained oligonucleotide base plane is presented from the surface in a manner effective to dissociably hybridize to a complementary single-stranded nucleic acid with asymmetric, substantially non-helical base pairing without alteration of the oligonucleotide base plane presentation and without oligonucleotide phosphate group dissociation from the surface.

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