Detection of nucleic acids from multiple types of human papillomaviruses
First Claim
1. A mixture of oligomers for detecting human papillomavirus (HPV) nucleic acid made up of amplification oligomers made up of first amplification oligomers of SEQ ID Nos. 18, 20, 22, 24, 26, 28, 30, 32, 34 and 36, sequences completely complementary to SEQ ID Nos. 18, 20, 22, 24, 26, 28, 30, 32, 34 and 36, or RNA equivalents of SEQ ID Nos. 18, 20, 22, 24, 26, 28, 30, 32, 34 and 36 and second amplification oligomers of SEQ ID Nos. 38, 39, 40 and 41, sequences completely complementary to SEQ ID Nos. 38, 39, 40 and 41, or RNA equivalents of SEQ ID Nos. 38, 39, 40 and 41.
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Abstract
Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed.
93 Citations
20 Claims
- 1. A mixture of oligomers for detecting human papillomavirus (HPV) nucleic acid made up of amplification oligomers made up of first amplification oligomers of SEQ ID Nos. 18, 20, 22, 24, 26, 28, 30, 32, 34 and 36, sequences completely complementary to SEQ ID Nos. 18, 20, 22, 24, 26, 28, 30, 32, 34 and 36, or RNA equivalents of SEQ ID Nos. 18, 20, 22, 24, 26, 28, 30, 32, 34 and 36 and second amplification oligomers of SEQ ID Nos. 38, 39, 40 and 41, sequences completely complementary to SEQ ID Nos. 38, 39, 40 and 41, or RNA equivalents of SEQ ID Nos. 38, 39, 40 and 41.
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12. A method of detecting human papillomavirus (HPV) nucleic acid present in a biological sample, comprising the steps of:
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contacting nucleic acid in a biological sample containing RNA of at least one of HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68 with amixture of amplification oligomers that amplify a HPV sequence in an E6/E7 target region sequence, in which the mixture is made up of first amplification oligomers consisting of SEQ ID Nos. 18, 20, 22, 24, 26, 28, 30, 32, 34 and 36, and second amplification oligomers consisting of SEQ ID Nos. 38, 39, 40 and 41, or completely complementary oligomer sequences or RNA equivalents of the first and second amplification oligomer sequences; amplifying a HPV sequence from the target region sequence in at least one HPV type by using the amplifcation oligomers and a nucleic acid polymerase in vitro to produce an HPV amplified product; and detecting the amplified product by using a detection probe oligomer that is sufficiently complementary to hybridize specifically with the HPV amplified product to indicate the presence in the sample oat least one of HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58,59 and 68. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20)
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Specification