PGC-1β, a novel PGC-1 homologue and uses therefor

• US 7,354,738 B2
• Filed: 04/27/2006
• Issued: 04/08/2008
• Est. Priority Date: 11/09/2001
• Status: Expired due to Term

• Current Assignee: Dana-Farber Cancer Institute
• Original Assignee: Dana-Farber Cancer Institute
• Inventors: Lin, Jiandie, Spiegelman, Bruce M.
• Primary Examiner(s): Saidha; Tekchand
• Application Number:
  US11/413,066
• Publication Number:
  US 20070009933A1
• Time in Patent Office: 712 Days
• Field of Search: 435/69.1, 435/252.3, 435/320.1, 536/23.1, 530/300, 530/350
• US Class Current: 435/69.1
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 25/16 Anti-Parkinson drugs
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: C07K 14/4705 stimulating, promoting or activating activity

Abstract

The invention provides isolated nucleic acid molecules, designated PGC-1β nucleic acid molecules, which encode novel PGC-1 related coactivator molecules. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing PGC-1β nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a PGC-1β gene has been introduced or disrupted. The invention still further provides isolated PGC-1β proteins, fusion proteins, antigenic peptides and anti-PGC-1β antibodies. Diagnostic and therapeutic methods utilizing compositions of the invention are also provided.

Claims

1. An isolated polypeptide comprising an amino acid sequence which is at least 97% identical to the amino acid sequence of SEQ ID NO:

5, wherein the polypeptide modulates one or more of the following biological activities;

activity of a nuclear receptor and/or nuclear respiratory factor 1 (NRF1);

brown adipose cell determination and/or differentiation;

mitochondrial activity and/or biogenesis;

or fatty acid β

-oxidation.

2. The isolated polypeptide of claim 1 comprising the amino acid sequence of SEQ ID NO:

5.

3. The polypeptide of claim 1, further comprising a heterologous amino acid sequence.

4. An isolated polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least 97% identical to the nucleic acid sequence of SEQ ID NO:

4 or 6, wherein the polypeptide modulates one or more of the following biological activities;

activity of a nuclear receptor and/or nuclear respiratory factor 1 (NRF1);

brown adipose cell determination and/or differentiation;

mitochondrial activity and/or biogenesis;

or fatty acid β

-oxidation.

5. The isolated polypeptide of claim 4, further comprising a heterologous amino acid sequence.