Oligonucleotides for use in determining the presence of human papilloma virus in a test sample
First Claim
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1. A hybridization assay probe for use in determining the presence of HPV Type 18 nucleic acid in a sample, wherein the base sequence of said probe is up to 100 bases in length and consists of a target binding region having no more than a 10% base difference with a base sequence selected from the group consisting of SEQ ID NO:
- 45, SEQ ID NO;
46, SEQ ID NO;
47 and SEQ ID NO;
48 and, optionally, additional nucleotides that are non-complementary to a nucleic acid derived from HPV Type 18 adjacent the complement of said target binding region, wherein said probe forms a detectable probe;
target duplex with a target nucleic acid sequence derived from HPV Type 18 under selective stringency hybridization conditions, and wherein said probe does not form a detectable probe;
non-target duplex with nucleic acid from HPV Type 16 under said conditions.
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Abstract
The present invention describes oligonucleotides targeted to HPV Type 16 and/or Type 18 nucleic acid sequences which are particularly useful to aid in detecting HPV type 16 and or 18. The oligonucleotides can aid in detecting HPV Type 16 and/or Type 18 in different ways such as by acting as hybridization assay probes, helper probes, and/or amplification primers.
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Citations
20 Claims
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1. A hybridization assay probe for use in determining the presence of HPV Type 18 nucleic acid in a sample, wherein the base sequence of said probe is up to 100 bases in length and consists of a target binding region having no more than a 10% base difference with a base sequence selected from the group consisting of SEQ ID NO:
- 45, SEQ ID NO;
46, SEQ ID NO;
47 and SEQ ID NO;
48 and, optionally, additional nucleotides that are non-complementary to a nucleic acid derived from HPV Type 18 adjacent the complement of said target binding region, wherein said probe forms a detectable probe;
target duplex with a target nucleic acid sequence derived from HPV Type 18 under selective stringency hybridization conditions, and wherein said probe does not form a detectable probe;
non-target duplex with nucleic acid from HPV Type 16 under said conditions. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
- 45, SEQ ID NO;
Specification