Method for the diagnosis and prognosis of malignant diseases

• US 7,357,928 B2
• Filed: 10/09/2003
• Issued: 04/15/2008
• Est. Priority Date: 04/08/2002
• Status: Expired due to Fees

• Current Assignee: University of Louisville
• Original Assignee: University of Louisville
• Inventors: Bates, Paula J., Xu, Xiaohua, Trent, John O., Miller, Donald M.
• Primary Examiner(s): Yaen; Christopher H.
• Application Number:
  US10/683,480
• Publication Number:
  US 20050053607A1
• Time in Patent Office: 1,650 Days
• Field of Search: None
• US Class Current: 424/130.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 39/39558 against tumor tissues, cells, antigens
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61N 5/10 X-ray therapy; Gamma-ray therapy; Particle-irradiation therapy A61N5/01 takes precedence
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: C12N 15/113 Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing when used in plants C12N15/8218
• CPC Class Codes: C12Q 1/6886 for cancer immunoassay for cancer G01N33/574
• CPC Class Codes: G01N 33/5005 involving human or animal cells immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025
• CPC Class Codes: G01N 33/57492 involving compounds localized on the membrane of tumor or cancer cells

Abstract

Methods for the treatment of tumors and cancer by exploiting the surface expression of the usually nuclear-localized protein, nucleolin.

Claims

1. A method of treating cancer in a mammal comprising administering to said mammal a therapeutically effective amount of an anti-nucleolin agent and a pharmaceutically acceptable carrier;

wherein the anti-nucleolin agent comprises a nucleolin monoclonal antibody.

2. The method of claim 1, further comprising administering to said mammal a chemotoxic or chemotherapeutic agent.

3. The method of claim 1, further comprising treating said mammal with radiation therapy.

4. The method of claim 2, wherein said chemotoxic or chemotherapeutic agent is selected from the group consisting of cyclophosphamide, etoposide, doxorubicin, methotrexate, vincristine, procabazine, prednizone, dexamethasone, tamoxifen citrate, carboplatin, cisplatin, oxaliplatin, 5-fluorouracil, camptothecin, zoledronic acid, Ibandronate and mytomycin.

12. The method of claim 2, further comprising treating said mammal with radiation therapy.

13. The method of claim 4, further comprising treating said mammal with radiation therapy.

18. The method of claim 1, wherein the therapeutically effective amount of an anti-nucleolin agent and a pharmaceutically acceptable carrier comprises an injectable formulation.

5. A method of treating cancer in a human, comprising administering to said human a therapeutically effective amount of an anti-nucleolin monoclonal antibody and a pharmaceutically acceptable carrier, wherein said nucleolin antibody is substantially non-immunogenic to human.

6. The method of claim 5, further comprising administering to said human a chemotoxic or chemotherapeutic agent.

7. The method of claim 5, further comprising treating said human with radiation therapy.

8. The method of claim 6 wherein said chemotoxic or chemotherapeutic agent is selected from the group consisting of cyclophosphamide, etoposide, doxorubicin, methotrexate, vincristine, procabazine, prednizone, dexamethasone, tamoxifen citrate, carboplatin, cisplatin, oxaliplatin, 5-fluorouracil, camptothecin, zoledronic acid, Ibandronate and mytomycin.

14. The method of claim 6, further comprising treating said human with radiation therapy.

15. The method of claim 8, further comprising treating said human with radiation therapy.

19. The method of claim 5, wherein the therapeutically effective amount of an anti-nucleolin monoclonal antibody and a pharmaceutically acceptable carrier comprises an injectable formulation.

9. A method of treating cancer in a mammal comprising administering to said mammal a therapeutically effective amount of an anti-nucleolin monoclonal antibody, a chemotoxic or chemotherapeutic agent and a pharmaceutically acceptable carrier.

10. The method of claim 9, wherein said chemotoxic or chemotherapeutic agent is selected from the group consisting of cyclophosphamide, etoposide, doxorubicin, methotrexate, vincristine, procabazine, prednizone, dexamethasone, tamoxifen citrate, carboplatin, cisplatin, oxaliplatin, 5-fluorouracil, camptothecin, zoledronic acid, Ibandronate and mytomycin.

16. The method of claim 9, further comprising treating said mammal with radiation therapy.

17. The method of claim 10, further comprising treating said mammal with radiation therapy.

20. The method of claim 9, wherein the therapeutically effective amount of an anti-nucleolin monoclonal antibody, a chemotoxic or chemotherapeutic agent and a pharmaceutically acceptable carrier comprises an injectable formulation.

11. A method of treating cancer in a mammal comprising administering to said mammal a therapeutically effective amount of an anti-nucleolin monoclonal antibody and a pharmaceutically acceptable carrier, and further treating said mammal with radiation therapy.

21. The method of claim 11, wherein the therapeutically effective amount of an anti-nucleolin monoclonal antibody and a pharmaceutically acceptable carrier comprises an injectable formulation.