Integrated system for on-site cell acquisition, processing, and delivery

• US 7,364,657 B2
• Filed: 03/31/2005
• Issued: 04/29/2008
• Est. Priority Date: 03/31/2005
• Status: Expired due to Fees

• Current Assignee: Abbott Cardiovascular Systems Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Abbott Cardiovascular Systems Incorporated (Abbott Laboratories Incorporated)
• Inventors: Michel, Eugene, Shen, Yan, Mandrusov, Evgenia, Chin, Albert K., Ainsworth, Robert D., Webler, William Earl Jr.
• Primary Examiner(s): KIM, SUN U
• Application Number:
  US11/097,022
• Publication Number:
  US 20060223176A1
• Time in Patent Office: 1,125 Days
• Field of Search: 210/85, 210/86, 210/87, 210/143, 210/198.1, 210/199, 210/201, 210/203, 210/252, 210/258, 210/359, 210/416.1, 435/2, 435/286.5, 435/287.1, 494/10, 494/37, 494/43, 494/50, 604/6.1, 604/6.11, 604/406
• US Class Current: 210/252
• CPC Class Codes: A61B 1/015 Control of fluid supply or evacuation
• CPC Class Codes: A61B 1/313 for introducing through surgical openings, e.g. laparoscopes
• CPC Class Codes: A61B 17/3472 for bones, e.g. intraosseus injections
• CPC Class Codes: A61B 2017/00969 used for transplantation
• CPC Class Codes: A61B 2217/005 with suction drainage system
• CPC Class Codes: A61K 2035/124 the cells being hematopoietic, bone marrow derived or blood cells
• CPC Class Codes: A61K 35/28 Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
• CPC Class Codes: A61M 2202/10 Bone-marrow
• CPC Class Codes: A61M 2210/02 Bones
• CPC Class Codes: A61M 2210/125 Heart
• CPC Class Codes: A61M 5/00 Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests vaccination appliances for veterinary use A61D1/025 ; tube connectors, tube couplings, valves or branch units specially adapted for medical use A61M39/00; containers specially adapted for medical or pharmaceutical purposes A61J1/00; combinations of vial and syringe for mixing or transferring their contents A61J1/20; holders for containers for collecting, storing or administering blood or medical fluids A61J1/16

Abstract

Numerous embodiments of a system and method for treating cardiac tissue are described. In one embodiment, bone marrow cells are extracted from a patient. The cells are then processed to isolate mononuclear cells, which can then be delivered back near the cardiac tissue of the patient.

Claims

1. A system, comprising;

a cell processing device to separate mononuclear cells from a bone marrow extract, the cell processing device comprising a first syringe coupled to a second syringe and a rotating mechanism coupled to the first syringe; and

a first container;

a gradient solution for the cell processing device in the first container;

a second container; and

a washing solution for the cell processing device in the second container,

the first container and the second container coupled to the cell processing device with a network of tubes.

2. The system of claim 1, wherein the network of tubes include a plurality of stopcocks to control a flow of fluid through the tubes.

3. The system of claim 1 further comprising a waste container coupled to the cell processing device for disposal of a waste fluid generated by the cell processing device.

4. The system of claim 3, wherein the waste container is coupled to the first syringe and the second syringe.

5. The system of claim 1, wherein a sensor mechanism is coupled to the cell processing device to detect fluids passing through the cell processing device.

6. The system of claim 5, wherein the sensor mechanism comprises an optical sensor to detect an amount of fluid in the first syringe.

7. The system of claim 1, wherein the cell processing device further comprises:

a port to receive the bone marrow extract, said port in fluid communication with the first syringe; and

a first filter disposed between the first container and the first syringe to remove debris from the bone marrow extract before the bone marrow extract is passed into the first syringe.

8. The system of claim 1, wherein the cell processing device includes a filter disposed between the second container and the second syringe.

9. The system of claim 1, wherein the cell processing device is disposable.

10. The system of claim 1, further comprising a tracking device coupled to the cell processing device to identify the cell processing device during use.

11. The system of claim 10, wherein the tracking device comprises a bar-code labeling system.

12. The system of claim 1, wherein the first syringe and the second syringe each include a plunger comprising an automated translation mechanism to control an amount of fluid contained in the first syringe and the second syringe.

13. The system of claim 1, wherein the rotating mechanism comprises a centrifugal mechanism.

14. A cell processing system, comprising:

a mixture comprising a density gradient solution, a washing solution, and a bone marrow extract;

a first syringe coupled to a secondary syringe of a processing set;

a filter coupled to the first syringe and the second syringe;

a washing solution;

means for drawing the mixture into the first syringe configured to separate mononuclear cells from a bone marrow extract;

means for spinning the first syringe to separate the mixture into a layer of mononuclear cells and a layer of waste fluid;

means for passing the layer of mononuclear cells through the filter coupled to the first syringe and the second syringe to collect the mononuclear cells in the filter;

means for drawing the washing solution through the filter and into the second syringe to wash out the mononuclear cells for collection of the mononuclear cells in the second syringe.

15. The cell processing system of claim 14, wherein means for drawing the mixture further comprises means for wetting the processing set in preparation for a separation of mononuclear cells from the bone marrow extract.

16. The cell processing system of claim 15, wherein means for wetting further comprises:

a density gradient solution in a first container;

a washing solution in a second container;

means for drawing the density gradient solution from the first container coupled to the first syringe, the first container to supply the density gradient solution; and

means for drawing the washing solution from the second container, the second syringe also coupled to the second container to supply the washing solution.

17. The cell processing system of claim 14, wherein means for drawing the mixture further comprises means for sensing an amount of the mixture drawn into the first syringe.

18. The cell processing system of claim 14 further comprising a bone marrow extract, wherein means for drawing the mixture further comprises means for passing the bone marrow extract through a filter to remove debris before forming the mixture in the first syringe.

19. The cell processing system of claim 14, wherein means for passing further comprises means for transferring the layer of mononuclear cells through a filter coupled to the first syringe to collect the mononuclear cells and to pass additional waste fluid to a waste container.

20. The cell processing system of claim 14 further comprising a plunger coupled to the second syringe, wherein means for drawing the washing solution further comprises means for translating a plunger coupled to the second syringe to control an amount of the washing solution passed through the filter and into the second syringe.

21. The cell processing system of claim 14, wherein the means for drawing the washing solution further comprises means for calculating an amount, volume, and count of the mononuclear cells.

22. The cell processing system of claim 14, wherein the means for drawing the washing solution further comprises means for identifying and labeling the mononuclear cells collected in the second syringe with a particular patient.