Treatment of substrates for immobilizing biomolecules

• US 7,364,849 B2
• Filed: 12/05/2003
• Issued: 04/29/2008
• Est. Priority Date: 08/09/2001
• Status: Expired due to Fees

• Current Assignee: Corning Incorporated
• Original Assignee: Corning Incorporated
• Inventors: Xie, Yuming, Raghavachari, Nalini, Xie, Xinying, Bao, Yijia, He, Mingqian, Li, Guangshan
• Primary Examiner(s): WHISENANT, ETHAN C
• Application Number:
  US10/729,619
• Publication Number:
  US 20040142365A1
• Time in Patent Office: 1,607 Days
• Field of Search: 435/6, 435/7.1, 536/23.1, 536/24.3, 530/350
• US Class Current: 435/6.11
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Abstract

A method of treating a substrate for immobilizing a biomolecule and substrates produced by the method are disclosed. The method includes contacting at least a portion of a substrate with a reducing agent such as a hydride. Treatment with an appropriate reducing agent substantially eliminates autofluorescence on substrates.

Claims

1. A method of non-covalently immobilizing biomolecules on a surface of an array substrate, wherein the array substrate comprises an inorganic material, comprising:

providing the array substrate having a first surface including a functional group for non-covalent attachment to a biomolecule;

contacting at least a portion of the first surface with a reducing agent;

attaching a biomolecule to the functional group after said contacting step whereby the biomolecule is non-covalently immobilized on a surface of said array.

2. The method of claim 1, wherein the reducing agent includes a hydride.

3. The method of claim 1, wherein the reducing agent includes a borohydride.

4. The method of claim 3, wherein the borohydride includes sodium borohydride.

5. The method of claim 4, wherein the sodium borohydride is in a solution at a concentration ranging from 0.01% to 1% by volume.

6. An array substrate made in accordance with the method of claim 2.

7. An array substrate made in accordance with the method of claim 5.

8. The method of claim 1, wherein the array substrate is coated with an amino-silane.

9. The method of claim 8, wherein the amino-silane includes gamma-amino-propyl-silane.

10. The method of claim 3, wherein the sodium borohydride is in a solution at a concentration ranging from 0.2% to 0.3% by volume.

11. The method of claim 1, wherein the reducing agent includes sodium cyanoborohydride, copper sulfate, or hydrogen.

12. The method of claim 1, wherein the array substrate comprises a microarray.

13. A method of non-covalently immobilizing biomolecules on a surface of a substrate comprising:

providing a substrate having a first surface including a functional group for non-covalent attachment to a biomolecule, wherein the substrate comprises an inorganic material;

contacting at least a portion of the first surface with a reducing agent;

attaching a biomolecule to the functional group after said contacting step whereby the biomolecule is non-covalently immobilized on a surface of said array.

14. The method of claim 13, wherein the inorganic material is a metal, a semiconductor material, a glass, or a ceramic material.

15. The method of claim 14, wherein the glass or ceramic material is quartz, glass, porcelain, alkaline earth aluminoborosilicate glass, or a mixed oxide.

16. The method of claim 13, wherein the substrate is a glass slide.

17. The method of claim 13, wherein the includes sodium borohydride.

18. A substrate made in accordance with the method of claim 13.

19. A method of immobilizing biomolecules on a surface of an array substrate, wherein the array substrate comprises an organic material, wherein the organic material comprises a polyester, a polyvinylchloride, a polyvinylidene fluoride, a polytetrafluoroethylene, a polycarbonate, a polyamide, a poly(meth)acrylate, a polystyrene, a polyethylene, or a ethylene/vinyl acetate copolymer, comprising:

providing the array substrate having a first surface including a functional group for non-covalent attachment to a biomolecule;

contacting at least a portion of the first surface with a reducing agent;

attaching a biomolecule to the functional group.