Multiplexed analytical platform
First Claim
1. A system for detecting one or more properties of each of a plurality of analytes, the system comprising:
- a first set of analyte-interaction nucleic acid moieties capable of reacting with the plurality of analytes under assay reaction conditions, each analyte-interaction nucleic acid moiety having a synthesis tag comprising a concatenation of words;
conversion reagents for converting each synthesis tag into a hybridization tag and for releasing the hybridization tag from the analyte-interaction nucleic acid moieties, wherein the hybridization tag comprises one or more words determined by a corresponding synthesis tag, each word of the hybridization tag being longer than each word of its corresponding synthesis tag; and
a solid phase detection array for detecting the released hybridization tags, thereby detecting the one or more properties of each of the plurality of analytes.
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Abstract
The invention provides a system and reagents for carrying out multiplexed analytical measurements using the hybridization of oligonucleotide tags to addressable solid phase supports to obtain simultaneous readouts from hundreds to tens of thousands of reactions. In one aspect of the invention, analyte interaction moieties, such as oligonucleotide probes, are produced that are each labeled with a unique synthesis tag constructed from a set of two-nucleotide “words.” During or after an assay, the synthesis tags are converted to hybridization tags that are used in the readout step. Hybridization tags are constructed to maximize discrimination in the readout. Hybridization tags of the invention are preferably constructed from oligonucleotide “words” selected from a minimally cross-hybridizing set using combinatorial synthesis techniques. In a further aspect, discrimination in the readout is enhanced by employing hybridization tags that are constructed with words having the “comma-less” property and by minimizing or eliminating extraneous nucleic acids associated with the hybridization tag during a readout step.
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Citations
6 Claims
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1. A system for detecting one or more properties of each of a plurality of analytes, the system comprising:
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a first set of analyte-interaction nucleic acid moieties capable of reacting with the plurality of analytes under assay reaction conditions, each analyte-interaction nucleic acid moiety having a synthesis tag comprising a concatenation of words; conversion reagents for converting each synthesis tag into a hybridization tag and for releasing the hybridization tag from the analyte-interaction nucleic acid moieties, wherein the hybridization tag comprises one or more words determined by a corresponding synthesis tag, each word of the hybridization tag being longer than each word of its corresponding synthesis tag; and a solid phase detection array for detecting the released hybridization tags, thereby detecting the one or more properties of each of the plurality of analytes. - View Dependent Claims (2, 3, 4, 5)
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6. A solid phase support having attached a plurality of tag complements, the solid phase support comprising:
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a plurality of addressable sites, each addressable site having copies of only one tag complement, the tag complement having the structure;
W1W2. . . Wnwherein each Wi for i=1, 2, . . . n is selected from a group of oligonucleotides having nucleotide sequences;
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Specification