Glyoxal/zinc fixative
First Claim
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1. A mercury-free, formaldehyde-free fixative composition for preserving a biological specimen, comprising:
- a. low molecular weight alcohol, which is present in the composition in an amount that is greater than 0 to about 300 milliliters per liter;
b. from about 10 grams per liter to about 100 grams per liter of polyvinyl alcohol;
c. from about 10 grams per liter to about 150 grams per liter of zinc sulfate;
d. from about 1 gram per liter to about 50 grams per liter of glyoxal;
e. from about 0.01 M per liter to about 1.0 M per liter of buffer; and
f. water.
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Abstract
This invention provides compositions and methods for fixing a biological sample, particularly fecal samples for diagnosis of parasitic infection. The fixative composition of the present invention comprises glyoxal (pyruvate aldehyde) and zinc sulfate and permits staining of biological samples without use of toxic compounds, such as formaldehyde and mercury-containing compounds. The fixative is compatible with many diagnostic assays, including trichrome stains, hematoxlin, ELISA, fluorescent assays, and lateral flow assays.
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18 Claims
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1. A mercury-free, formaldehyde-free fixative composition for preserving a biological specimen, comprising:
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a. low molecular weight alcohol, which is present in the composition in an amount that is greater than 0 to about 300 milliliters per liter; b. from about 10 grams per liter to about 100 grams per liter of polyvinyl alcohol; c. from about 10 grams per liter to about 150 grams per liter of zinc sulfate; d. from about 1 gram per liter to about 50 grams per liter of glyoxal; e. from about 0.01 M per liter to about 1.0 M per liter of buffer; and f. water. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A formaldehyde-free, mercury-free fixative composition for preserving biological specimens, comprising:
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a. from about 200 milliliters per liter to about 250 milliliters per liter of low molecular weight alcohol; b. from about 25 grams per liter to about 75 grams per liter of polyvinyl alcohol; c. from about 50 grams per liter to about 100 grams per liter of zinc sulfate; d. from about 5 grams per liter to about 25 grams per liter of glyoxal; e. from about 0.05 M per liter to about 0.5 M per liter of buffer; and f. water, wherein the pH of the fixative composition is from about 3.5 to about 5.0 - View Dependent Claims (15)
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16. A formaldehyde-free, mercury-free fixative composition for preserving biological specimens, comprising:
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a. about 225 milliliters per liter of low molecular weight alcohol; b. about 40 grams per liter of polyvinyl alcohol; c. about 75 grams per liter of zinc sulfate; d. about 12 grams per liter of glyoxal; e. about 0.10 M per liter of buffer; and f. water. - View Dependent Claims (17, 18)
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Specification