Process of isolating lactoferrin

• US 7,368,141 B2
• Filed: 03/06/2003
• Issued: 05/06/2008
• Est. Priority Date: 03/07/2002
• Status: Active Grant

• Current Assignee: Upfront Chromatography A/S
• Original Assignee: Upfront Chromatography A/S
• Inventors: Lihme, Allan Otto Fog
• Primary Examiner(s): NAVARRO, ALBERT MARK
• Application Number:
  US10/507,136
• Publication Number:
  US 20050220953A1
• Time in Patent Office: 1,888 Days
• Field of Search: 426/531, 426/665, 530/350
• US Class Current: 426/531
• CPC Class Codes: A23J 1/202 Casein or caseinates
• CPC Class Codes: A23J 1/207 Co-precipitates of casein and lactalbumine

Abstract

A process for isolating lactoferrin from milk or a casein-containing component or product containing lactoferrin, comprising subjecting a precipitate of casein obtained from the milk of the casein-containing component or product, with which precipitate at least part of the milk lactoferrin is associated, to a treatment with an extraction medium whereby at least part of the lactoferrin associated with the precipitate of casein is released to the medium. When lactoferrin has been released, the medium may be subjected to different types of purification operation steps. The purification operations are selected in accordance with the purity requirements of the intended specific application. Furthermore, lactoferrin and/or a composition comprising lactoferrin according to the above-mentioned process is provided.

Claims

1. A process for isolating lactoferrin comprising:

i. precipitating a casein precipitate from a product that comprises lactoferrin to a casein precipitate, whereby said lactoferrin associates with said casein precipitate to form a lactoferrin-casein adsorption product; and

ii. extracting said lactoferrin-casein adsorption complex with an extraction medium, thereby releasing at least part of said lactoferrin from said lactoferrin-casein adsorption product into the extraction medium.

2. The process of claim 1, wherein said casein precipitate is obtained from milk, and wherein said product that comprises lactoferrin is milk, and further comprising after step (i) and before step (ii) the step of washing said lactoferrin-casein adsorption product with a washing solution to wash out at least part of undesired entrained whey proteins from the casein precipitate.

3. The process of claim 2, wherein the washing solution is drained off the casein precipitate.

4. The process of claim 1, wherein the lactoferrin containing extraction medium is separated from the casein precipitate by at least one method selected from the group consisting of draining, centrifugation, filtration and filter pressing.

5. The process of claim 1, wherein the extraction medium provides a change in at least one parameter selected from the group consisting of pH, ionic strength, salt content, hydrophobicity, charge, temperature and pressure.

6. The process of claim 1, wherein the extraction medium is at least one member selected from the group consisting of inorganic salts, organic salts, acids, and bases.

7. The process of claim 1, wherein the extraction medium comprises a final salt concentration in the range of 0.01-5N.

8. The process of claim 1, wherein the extraction medium has a pH in the range of 1-5.

9. The process of claim 1, wherein said product containing lactoferrin is obtained from an animal.

10. The process of claim 2 wherein said milk is obtained from a transgenic animal producing human or animal lactoferrin in their milk.

11. The process of claim 1, wherein the animal is selected from the group consisting of herd animals, cows, camels, buffaloes, pigs, horses, deer, sheep, goats, dogs and cats.

12. The process of claim 1, wherein lactoferrin is isolated by a batch operation or by continuous operation.

13. The process of claim 4, wherein the lactoferrin in said extraction medium separated from said casein precipitate is further isolated by at lease one method selected from the group consisting of clarification, concentration and purification.

14. The process of claim 13, wherein said further isolated lactoferrin is clarified by at least one method selected from the group consisting of filtration, membrane filtration, centrifugation and sedimentation.

15. The process of claim 13 or 14, wherein lactoferrin is concentrated by at least one method selected from the group consisting of membrane filtration, ultrafiltration, nanofiltration, reversed osmosis, evaporation and precipitation.

16. The process of claim 15, wherein said concentrated lactoferring is purified by at least one adsorption technique selected from the group consisting of ion exchange, hydrophobic adsorption, affinity adsorption, mixed mode ligand adsorption, metal chelate adsorption, reversed phase adsorption, gel filtration and precipitation.

17. The process of claim 16, wherein the adsorption technique is performed in an expanded bed mode, a packed bed mode or both.

18. The process of claim 16, wherein said lactoferrin is adsorbed by a resin at a pH in the range of 3-7.

19. The process of claim 16, wherein said lactoferrin is eluted from a resin by increasing pH.

20. The process of claim 19, wherein the pH for elution of said lactoferrin adsorbed to a resin is in the range of 8-13.

21. The process of claim 1, wherein said casein precipitate is obtained from dairy product.

22. The process of claim 1, wherein said lactoferrin is isolated from a casein precipitate obtained from a casein and/or caseinate production.

23. The process of claim 1, wherein said lactoferrin is isolated from a casein precipitate obtained from a cheese production.

24. A lactoferrin enriched extract comprising lactoferrin isolated according to the process of claim 1.

25. A composition comprising the lactoferrin enriched extract of claim 24.

26. A method for preserving food products comprising combining the lactoferrin enriched extract of claim 24 with a food product.

27. The method of claim 26, further comprising subjecting said combined lactoferrin enriched extract to a preparation step.

28. The method of claim 26 or 27, wherein said food product is a meat product.