Multiple array format

• US 7,371,348 B2
• Filed: 06/14/2002
• Issued: 05/13/2008
• Est. Priority Date: 06/14/2002
• Status: Expired due to Term

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies Europe BV (Agilent Technologies, Inc.)
• Inventors: Caren, Michael P, Schleifer, Arthur
• Primary Examiner(s): WALLENHORST, MAUREEN
• Application Number:
  US10/173,292
• Publication Number:
  US 20030231989A1
• Time in Patent Office: 2,160 Days
• Field of Search: 422/99, 422/102, 422/104, 422/939, 435/283.1, 435/287.1, 435/288.3, 435/288.4, 435/305.4, 359/398, 264/494
• US Class Current: 422/559
• CPC Class Codes: B01J 2219/00351 Means for dispensing and evacuation of reagents
• CPC Class Codes: B01J 2219/00662 Two-dimensional arrays within two-dimensional arrays
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/0822 Slides
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: C40B 60/14 for creating libraries

Abstract

A multiple array substrate having multiple assay chambers, each assay chamber containing one or more arrays. The assay chambers include a form-in-place gasket between a substrate and a cover. Methods of forming such multiple array substrates are described. The assay chambers may further be associated with an analysis site for analyzing a sample solution contained within the assay chamber. Each assay chamber is in fluid communication with a port, and the ports are positioned in a spatial format adapted to interface to standard laboratory equipment for handling multiple fluids in parallel.

Claims

1. An apparatus comprising a plurality of assay chambers, the apparatus comprising, in order, a substrate, a form-in-place gasket, and a cover, wherein the form-in-place gasket, the substrate, and the cover together substantially define a plurality of assay chambers, wherein the form-in-place gasket forms a permanent seal between the form-in-place gasket and the cover producing a plurality of fluid containment structures, each fluid containment structure housing at least one assay chamber;

and the apparatus further comprises a plurality of arrays, wherein each array being in operable association with one or more of the assay chambers, wherein each fluid containment structure is contained on the top by the cover, wherein each fluid containment structure is contained on the side by the form-in-place gasket, wherein each fluid containment structure is contained on the bottom by the substrate, wherein the form-in-place gasket, the substrate, and the cover together further define a plurality of inlets and outlets, wherein the inlets and outlets are through the form-in-place gasket in a plane parallel with the substrate and the cover, wherein the inlet and the outlet are disposed on opposite ends of each assay chamber, wherein each assay chamber is in fluid communication with an inlet and an outlet, wherein the form-in-place gasket is between about 10 micrometers and about 250 micrometers thick, wherein the form-in-place gaskets consists of gasket materials selected from at least the following;

silicon sealants, urethanes, polysulfides, latex, and acrylic sealants, and wherein the cover and the substrate are made of different materials than the form-in-place gasket.

2. The apparatus of claim 1, wherein the assay chambers are spaced at uniform intervals.

3. The apparatus of claim 2, wherein the uniform interval is selected from the group consisting of about 4.5 mm, about 9 mm, and about 2.25 mm.

4. The apparatus of claim 3, wherein the inlets and outlets are in fluid communication with an external fluid dispensing system that handles multiple fluids in parallel.

5. The apparatus of claim 4, wherein the form-in-place gasket is between about 10 micrometers and about 100 micrometers thick.

6. The apparatus of claim 4, wherein the form-in-place gasket is between about 10 micrometers and about 50 micrometers thick.

7. The apparatus of claim 1, wherein the inlets are spaced at uniform intervals.

8. The apparatus of claim 7, wherein the uniform interval is selected from the group consisting of about 4.5 mm, about 9 mm, and about 2.25 mm.

9. The apparatus of claim 1, wherein the form-in-place gasket is between about 100 micrometers and about 3 millimeters wide.

10. An apparatus comprising a plurality of assay chambers, the apparatus comprising, in order, a substrate, a form-in-place gasket, and a cover, wherein the form-in-place gasket, the substrate, and the cover together substantially define a plurality of assay chambers, wherein the form-in-place gasket forms a permanent seal between the form-in-place gasket and the cover producing a plurality of fluid containment structures, each fluid containment structure housing at least one assay chamber, wherein each fluid containment structure is contained on the top by the cover, wherein each fluid containment structure is contained on the side by the form-in-place gasket, wherein each fluid containment structure is contained on the bottom by the substrate, wherein the form-in-place gasket, the substrate, and the cover together further define a plurality of inlets and outlets, wherein the inlets and outlets are through the form-in-place gasket in a plane parallel with the substrate and the cover, wherein the inlet and the outlet are disposed on opposite ends of each assay chamber, wherein each assay chamber is in fluid communication with an inlet and an outlet, wherein the assay chambers are spaced at uniform intervals, wherein a pair of the assay chambers are in fluidic communication via a channel, wherein the pair of assay chambers are positioned in series with one another, wherein the assay chambers are in fluid communication with an external fluid dispensing system, wherein the form-in-place gasket is between about 10 micrometers and about 250 micrometers thick, wherein the form-in-place gaskets consists of gasket materials selected from at least the following:

silicon sealants, urethanes, polysulfides, latex, and acrylic sealants, and wherein the cover and the substrate are made of different materials than the form-in-place gasket.

11. The apparatus of claim 10, wherein the inlets are fluidically coupled to an external fluid dispensing system that handles multiple fluids in parallel.

12. The apparatus of claim 11, wherein the inlets are spaced at uniform intervals.

13. The apparatus of claim 12, wherein the uniform interval is selected from the group consisting of about 4.5 mm, about 9 mm, and about 2.25 mm.

14. The apparatus of claim 13, further comprising a plurality of analysis components, each analysis component being in operable association with one or more of the assay chambers.

15. The apparatus of claim 14, wherein the analysis components are arrays, and wherein each assay chamber is in operable association with one or more of the arrays.

16. The apparatus of claim 13, wherein the form-in-place gasket is between about 10 micrometers and about 100 micrometers thick.

17. The apparatus of claim 10, wherein the form-in-place gasket is between about 10 micrometers and about 100 micrometers thick.

18. The apparatus of claim 10, wherein the form-in-place gasket is between about 100 micrometers and about 3 millimeters wide.

19. An apparatus comprising a plurality of assay chambers, the apparatus comprising, in order, a substrate, a form-in-place gasket, and a cover wherein the form-in-place gasket, the substrate, and the cover together substantially define a plurality of assay chambers, wherein the form-in-place gasket forms a permanent seal between the form-in-place gasket and the cover producing a plurality of fluid containment structures, each fluid containment structure housing at least one assay chamber, wherein each fluid containment structure is contained on the top by the cover, wherein each fluid containment structure is contained on the side by the form-in-place gasket, wherein each fluid containment structure is contained on the bottom by the substrate, wherein the form-in-place gasket, the substrate, and the cover together further define a plurality of inlets and outlets, each assay chamber in fluid communication with an inlet and an outlet, wherein the inlets and outlets are through the form-in-place gasket, wherein the inlet and the outlet are disposed on opposite ends of each assay chamber, wherein the inlets are coupled to an external fluid dispensing system, wherein the inlets are spaced at uniform intervals, wherein the form-in-place gasket is between about 10 micrometers and about 250 micrometers thick, and wherein the form-in-place gasket is formed from gasket materials selected from at least the following:

silicon sealants, urethanes, polysulfides, latex, and acrylic sealants, and wherein the cover and the substrate are made of different materials than the form-in-place gasket.

20. The apparatus of claim 19, wherein the assay chambers are spaced at uniform intervals.

21. The apparatus of claim 20, wherein the uniform interval is selected from the group consisting of about 4.5 mm, about 9 mm, and about 2.25 mm.

22. The apparatus of claim 19, further comprising a plurality of analysis components, each analysis component being in operable association with one or more of the assay chambers.

23. The apparatus of claim 22, wherein the analysis components are arrays, and wherein each assay chamber is in operable association with one or more of the arrays.

24. The apparatus of claim 19, wherein the form-in-place gasket is between about 10 micrometers and about 100 micrometers thick.

25. The apparatus or claim 19, wherein the form-in-place gasket is between about 10 micrometers and about 50 micrometers thick.

26. The apparatus of claim 19, wherein the form-in-place gasket is between about 100 micrometers and about 3 millimeters wide.

27. The apparatus of claim 19, wherein a pair of the assay chambers are in fluidic communication via a channel, wherein the pair of assay chambers are positioned in series with one another.