High purity X-chromosome bearing and Y-chromosome bearing populations of spermatozoa
First Claim
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1. A method of isolating X-chromosome bearing sperm cells and Y-chromosome bearing sperm cells, comprising the steps of:
- collecting viable sperm cells from a male of a species of non-human mammal;
staining said viable sperm cells to produce viable, stained sperm cells;
differentiating between X-chromosome bearing sperm cells and Y-chromosome bearing sperm cells based upon fluorescent light emitted from said viable, stained sperm cellswhich is detected with a photomultiplier tube operating at below 400 volts;
separating said X-chromosome bearing sperm cells and Y-chromosome bearing sperm cells into a X-chromosome bearing population and a Y-chromosome bearing population at a separation rate between 4,000 to 10,000 separations per second of each sex;
and producing separate X-chromosome bearing and Y-chromosome bearing populations of sperm cells each having a purity of greater than 90%.
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Abstract
Isolated non-naturally occurring populations of spermatozoa (15) having high purity and technologies to differentiate spermatozoa (28) based on characteristics such as mass, volume, orientation, or emitted light including methods of analysis and apparatus such as beam shaping optics (30) and detectors (32).
371 Citations
22 Claims
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1. A method of isolating X-chromosome bearing sperm cells and Y-chromosome bearing sperm cells, comprising the steps of:
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collecting viable sperm cells from a male of a species of non-human mammal; staining said viable sperm cells to produce viable, stained sperm cells; differentiating between X-chromosome bearing sperm cells and Y-chromosome bearing sperm cells based upon fluorescent light emitted from said viable, stained sperm cells which is detected with a photomultiplier tube operating at below 400 volts; separating said X-chromosome bearing sperm cells and Y-chromosome bearing sperm cells into a X-chromosome bearing population and a Y-chromosome bearing population at a separation rate between 4,000 to 10,000 separations per second of each sex; and producing separate X-chromosome bearing and Y-chromosome bearing populations of sperm cells each having a purity of greater than 90%. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification