×

Methods and kits for indirect labeling of nucleic acids

  • US 7,371,519 B2
  • Filed: 05/06/2003
  • Issued: 05/13/2008
  • Est. Priority Date: 01/31/2000
  • Status: Expired due to Term
First Claim
Patent Images

1. A method for comparing the expression profiles of at least two distinct samples, said method comprising:

  • (a) enzymatically generating;

    (i) a first population of oligonucleotide tagged nucleic acids of differing sequence from an mRNA sample derived from a first sample, wherein each oligonucleotide tagged nucleic acid of said first population comprises a same first oligonucleotide tag; and

    (ii) a second population of oligonucleotide tagged nucleic acids of differing sequence from an mRNA sample derived from a second sample, wherein each oligonucleotide tagged nucleic acid of said second population comprises a same second oligonucleotide tag that differs from said first oligonucleotide tag;

    (b) hybridizing said first and second populations of oligonucleotide tagged target nucleic acids to an array of probe nucleic acids stably associated with the surface of a solid support, wherein said hybridizing occurs in the presence of;

    (i) first labeled oligonucleotide complementary to said first oligonucleotide tag of said first population; and

    (ii) second labeled oligonucleotide complementary to said second oligonucleotide tag of said second population;

    whereby hybridized complexes comprising said oligonucleotide tagged target nucleic acids, probe nucleic acids and labeled oligonucleotides are produced on the surface of said array;

    (c) detecting the presence of said hybridized complexes on said array surface;

    (d) deriving an expression profile for each of said samples from said detected hybridized complexes; and

    (e) comparing the derived expression profiles of each of said samples;

    whereby the expression profiles of said at least two distinct samples are compared.

View all claims
  • 0 Assignments
Timeline View
Assignment View
    ×
    ×