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Methods and apparati using single polymer analysis

  • US 7,371,520 B2
  • Filed: 05/28/2003
  • Issued: 05/13/2008
  • Est. Priority Date: 05/28/2002
  • Status: Expired due to Fees
First Claim
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1. A method for analyzing a single nucleic acid molecule comprisingexposing a single nucleic acid molecule to at least two distinguishable detectable labels present on different unit specific markers for a time sufficient to allow the detectable labels to bind to the single nucleic acid molecule, andpassing the single nucleic acid molecule in flow through a diffraction-limited detection spot, thereby analyzing the single nucleic acid molecule for a coincident event using a single molecule detection system by detecting two separate and distinguishable signals from the at least two distinguishable detectable labels,wherein the single nucleic acid molecule is present at a concentration of 400 fM or greater, the coincidence event indicates that the at least two distinguishable detectable labels are bound to the single nucleic acid molecule, the coincident event is a color coincident event, and the method analyzes 20-20,000 single nucleic acid molecules per minute.

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