Method and apparatus for determining base sequence of nucleic acid molecule
First Claim
1. A method for determining the base sequence of a nucleic acid molecule, wherein the nucleic acid molecule is immobilized on a cantilever comprising a piezoelectric element, said method comprisinga) measuring the frequency of vibration of the piezoelectric element;
- b) cleaving the nucleic acid molecule;
c) measuring the frequency of vibration of the piezoelectric element; and
d) determining the sequence of the nucleic acid molecule by comparing the frequency measured in a) with the frequency measured in c).
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Abstract
Disclosed herein is a method and apparatus for determining the base sequence of a nucleic acid molecule by cleaving a nucleic acid molecule of interest while controlling the cleavage site, measuring the change in mass which occurs in the nucleic acid molecule after the cleavage step, and acquiring the base information of the cleaved nucleic acid molecule from the data about the change in mass. The method and apparatus are based on the principle which is entirely different from that used for the conventional technique.
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Citations
14 Claims
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1. A method for determining the base sequence of a nucleic acid molecule, wherein the nucleic acid molecule is immobilized on a cantilever comprising a piezoelectric element, said method comprising
a) measuring the frequency of vibration of the piezoelectric element; -
b) cleaving the nucleic acid molecule; c) measuring the frequency of vibration of the piezoelectric element; and d) determining the sequence of the nucleic acid molecule by comparing the frequency measured in a) with the frequency measured in c). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification