Microluminometer chip and method to measure bioluminescence

• US 7,371,538 B2
• Filed: 06/14/2005
• Issued: 05/13/2008
• Est. Priority Date: 11/25/1997
• Status: Expired due to Fees

• Current Assignee: UT-Battelle LLC
• Original Assignee: UT-Battelle LLC
• Inventors: Paulus, Michael J., Ripp, Steven A., Sayler, Gary S., Applegate, Bruce M., Simpson, Michael L.
• Primary Examiner(s): Chin; Christopher L.
• Application Number:
  US11/151,964
• Publication Number:
  US 20060281067A1
• Time in Patent Office: 1,064 Days
• Field of Search: 356/218, 356/226, 422/52, 422/55, 422/82.05, 422/82.08, 435/7.32, 435/288.7, 436/164, 436/172, 436/805
• US Class Current: 435/7.32
• CPC Class Codes: A61B 5/0031 Implanted circuitry
• CPC Class Codes: C12Q 1/02 involving viable microorganisms
• CPC Class Codes: C12Q 1/66 involving luciferase
• CPC Class Codes: G01N 21/763 Bioluminescence
• CPC Class Codes: G01N 33/74 involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 435/849 Escherichia coli
• CPC Class Codes: Y10S 435/879 Salmonella
• CPC Class Codes: Y10S 435/909 Vibrio
• CPC Class Codes: Y10S 436/805 Optical property

Abstract

An integrated microluminometer includes an integrated circuit chip having at least one n-well/p-substrate junction photodetector for converting light received into a photocurrent, and a detector on the chip for processing the photocurrent. A distributed electrode configuration including a plurality of spaced apart electrodes disposed on an active region of the photodetector is preferably used to raise efficiency.

Claims

1. An integrated microluminometer, comprising:

an integrated circuit chip that includes at least one n-well/p-substrate junction photodetector for converting light received into a photocurrent, and a detector on said chip for processing said photocurrent.

2. The integrated microluminometer of claim 1, wherein said photodetector includes a distributed electrode configuration comprising a plurality of spaced apart electrodes disposed on an active region of said photodetector.

3. The integrated microluminometer of claim 1, wherein said detector comprises a current-to-frequency converter.

4. The integrated microluminometer of claim 1, wherein said detector comprises an analog gated integrator.

5. The integrated microluminometer of claim 4, further comprising a discriminator connected to an output of said gated integrator, wherein said discriminator converts said photocurrent into a train of digital pulses, said pulses being are counted for a fixed time, wherein a digital word is generated which is proportional to said photocurrent.

6. The integrated microluminometer of claim 5, wherein said discriminator comprises a comparator coupled to a one-shot, said one-shot coupled to a gated counter, wherein an output of said gated counter is said digital word.

10. A method of measuring bioluminescence, comprising the steps of:

providing the microluminometer of claim 3 having a modified bioluminescent microorganism that emits light in the presence of a selected analyte held on a surface of said chip;

contacting said modified bioluminescent microorganism with a sample suspected of containing said analyte, and

operating said microluminometer of claim 3 and counting light pulses produced for a fixed time to determine said photocurrent, wherein said photocurrent is proportional to number of pulses that measure bioluminescence when said analyte causes said bioluminescent microorganism to emit said light.

11. The method of claim 10, wherein said modified bioluminescent microorganism is encapsulated in a sol-gel matrix held on said surface.

7. An integrated microluminometer, comprising:

a light detection system including at least one p-n junction based photodetector for converting light into electrical current and circuitry for reducing or canceling recombination and generation current produced by said photodetector.

8. The integrated microluminometer of claim 7, wherein said circuitry for reducing or canceling recombination and generation current comprises at least one diode in a feedback loop having an area smaller than an area of said photodetector, said diode supplying forward bias current that cancels out photocurrent associated with said recombination and said generation current.

9. The integrated microluminometer of claim 7, wherein said circuitry for reducing or canceling recombination and generation current maintains said photodiode at essentially zero bias.