FLP-mediated gene modification in mammalian cells, and compositions and cells useful therefor
First Claim
1. A co-transfection assay for the occurrence of FLP-mediated recombination, said assay comprising:
- (a) co-transfecting a host mammalian cell with;
(i) a FLP expression plasmid comprising a promoter operably linked to a gene encoding FLP recombinase wherein said promoter is active in said cell, and(ii) a reporter plasmid comprising a nonfunctional reporter gene wherein said nonfunctional reporter gene is inactivated by the presence of extraneous DNA flanked by FLP recombination target sites; and
(b) monitoring said host cell under a variety of conditions for the gain of expression of functional reporter gene product.
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Abstract
A gene activation/inactivation and site-specific integration system has been developed for mammalian cells. The invention system is based on the recombination of transfected sequences by FLP, a recombinase derived from Saccharomyces. In several cell lines, FLP has been shown to rapidly and precisely recombine copies of its specific target sequence. For example, a chromosomally integrated, silent β-galactosidase reporter gene was activated for expression by FLP-mediated removal of intervening sequences to generate clones of marked cells. Alternatively, the reverse reaction can be used to target transfected DNA to specific chromosomal sites. These results demonstrate that FLP can be used, for example, to mosaically activate or inactivate transgenes for a variety of therapeutic purposes, as well as for analysis of vertebrate development. The FLP recombination system of the present invention can be incorporated in transgenic, non-human mammals to achieve site-specific integration of transgenes, to construct functional genes or to disrupt existing genes.
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Citations
10 Claims
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1. A co-transfection assay for the occurrence of FLP-mediated recombination, said assay comprising:
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(a) co-transfecting a host mammalian cell with; (i) a FLP expression plasmid comprising a promoter operably linked to a gene encoding FLP recombinase wherein said promoter is active in said cell, and (ii) a reporter plasmid comprising a nonfunctional reporter gene wherein said nonfunctional reporter gene is inactivated by the presence of extraneous DNA flanked by FLP recombination target sites; and (b) monitoring said host cell under a variety of conditions for the gain of expression of functional reporter gene product. - View Dependent Claims (3, 4, 5, 6)
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2. A co-transfection assay for the occurrence of FLP-mediated recombination, said assay comprising:
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(a) co-transfecting a host mammalian cell with; (i) a FLP expression plasmid comprising a first promoter operably linked to a gene encoding FLP recombinase wherein said first promoter is active in said cell, and (ii) a reporter plasmid comprising a functional reporter gene operably linked to a second promoter active in said cell wherein said reporter gene comprises at least one FLP recombination target site therein; and (b) monitoring said host cell under a variety of conditions for the loss of expression of functional reporter gene product. - View Dependent Claims (7, 8, 9, 10)
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Specification