Bis-transition-metal-chelate probes
First Claim
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1. A method for detecting one or more molecules that include a target sequence, wherein said target sequence comprises an amino acid sequence of the form (H)i, wherein H is histidine and i is an integer of from 4 to 12, said method comprising:
- (a) providing a sample that comprises one or more target material, wherein said target material includes molecules having a target sequence,(b) subjecting said target material to electrophoresis in an electrophoretic medium;
(c) contacting said electrophoretic medium with at least one molecule having the general structural Formula (I), and tautomers, salts and acids thereof;
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Abstract
A molecule for labeling a target material is provided including two transition-metal chelates and a detectable group. The molecule has the general structural formula (I):
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- wherein: (a) Y and Y′ are each a transition metal, (b) R1 and R1′ are each independently CH(COO−), CH(COOH), or absent; (c) R2 and R2′ are linkers each having a length of from about 3.0 to about 20 Å; and (d) X is a detectable group. The linkers may be linear or branched, may contain aromatic moieties, and may optionally be further substituted. Methods of using the molecules of the invention as probes in detecting and analyzing target materials as well as kits including the molecule of the invention are also provided.
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Citations
20 Claims
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1. A method for detecting one or more molecules that include a target sequence, wherein said target sequence comprises an amino acid sequence of the form (H)i, wherein H is histidine and i is an integer of from 4 to 12, said method comprising:
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(a) providing a sample that comprises one or more target material, wherein said target material includes molecules having a target sequence, (b) subjecting said target material to electrophoresis in an electrophoretic medium; (c) contacting said electrophoretic medium with at least one molecule having the general structural Formula (I), and tautomers, salts and acids thereof; - View Dependent Claims (2, 4, 5, 6, 7, 8, 9, 10, 11)
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- 3. A solution for staining target molecules in an electrophoretic medium, said solution having an alkaline pH and comprising one or more molecules according to Formula (I), and tautomers, salts and acids thereof:
Specification