Bis-transition-metal-chelate probes

• US 7,371,745 B2
• Filed: 09/21/2004
• Issued: 05/13/2008
• Est. Priority Date: 03/28/2002
• Status: Expired due to Fees

• Current Assignee: Rutgers University
• Original Assignee: Rutgers University
• Inventors: Ebright, Yon W., Ebright, Richard H.
• Primary Examiner(s): SOLOLA, TAOFIQ A
• Application Number:
  US10/946,786
• Publication Number:
  US 20050031545A1
• Time in Patent Office: 1,330 Days
• Field of Search: 514/229.5, 514/338, 544/64
• US Class Current: 514/229.5
• CPC Class Codes: C07D 209/14 Radicals substituted by nitrogen atoms, not forming part of a nitro radical
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 2563/113 the label being electroactive, e.g. redox labels
• CPC Class Codes: G01N 33/533 with fluorescent label

Abstract

A molecule for labeling a target material is provided including two transition-metal chelates and a detectable group. The molecule has the general structural formula (I):

• • wherein: (a) Y and Y′ are each a transition metal, (b) R¹ and R^1′ are each independently CH(COO⁻), CH(COOH), or absent; (c) R² and R^2′ are linkers each having a length of from about 3.0 to about 20 Å; and (d) X is a detectable group. The linkers may be linear or branched, may contain aromatic moieties, and may optionally be further substituted. Methods of using the molecules of the invention as probes in detecting and analyzing target materials as well as kits including the molecule of the invention are also provided.

Claims

1. A method for detecting one or more molecules that include a target sequence, wherein said target sequence comprises an amino acid sequence of the form (H)_i, wherein H is histidine and i is an integer of from 4 to 12, said method comprising:

(a) providing a sample that comprises one or more target material, wherein said target material includes molecules having a target sequence,

(b) subjecting said target material to electrophoresis in an electrophoretic medium;

(c) contacting said electrophoretic medium with at least one molecule having the general structural Formula (I), and tautomers, salts and acids thereof;

2. The method of claim 1, wherein said electrophoresis is selected from the group consisting of solution electrophoresis, SDS-PAGE, IEF, IPG electrophoresis, and 2D electrophoresis.

4. The method of claim 1, wherein the electrophoretic medium is contacted with the at least one molecule according to Formula (I) in a solution having a pH of between 7.1 and 8.0.

5. The method of claim 1, wherein the electrophoretic medium is contacted with the at least one molecule according to Formula (I) in a solution having a pH of between 7.6 and 8.2.

6. The method of claim 1, wherein the electrophoretic medium is contacted with the at least one molecule according to Formula (I) in a solution having a pH of between 7.6 and 8.0.

7. The method of claim 4, wherein the at least one molecule according to Formula (I) is present at a concentration from 0.05 μ

M to 1 μ

M.

8. The method of claim 4, wherein said solution further comprises a preservative.

9. The method of claim 4, wherein said solution further comprises a phosphate buffer or a Tris buffer.

10. The method of claim 1, wherein the electrophoretic medium is contacted with the at least one molecule according to Formula (I) in a solution comprising a phosphate buffer.

11. The method of claim 4, wherein said solution further comprises a metal ion salt.

3. A solution for staining target molecules in an electrophoretic medium, said solution having an alkaline pH and comprising one or more molecules according to Formula (I), and tautomers, salts and acids thereof:

12. The solution of claim 3, wherein the solution has a pH of between 7.1 and 8.0.

13. The solution of claim 3, wherein the solution has a pH of between 7.6 and 8.2.

14. The solution of claim 3, wherein the solution has a pH of between 7.6 and 8.0.

15. The solution of claim 12, wherein the at least one molecule according to Formula (I) is present at a concentration from 0.05 μ

M to 1 μ

M.

16. The solution of claim 12, further comprising a preservative.

17. The solution of claim 12, further comprising a phosphate buffer or a Tris buffer.

18. The solution of claim 3, wherein the solution comprises a phosphate buffer.

19. The solution of claim 12, further comprising a metal ion salt.

20. The solution of claim 12, wherein the solution is present in a ready-to-use form in a container.