Multi-test analysis of real-time nucleic acid amplification
First Claim
1. A device for determining the presence of a nucleic acid in a sample comprisingan instrument for temperature cycling for analysis of real-time amplification of the nucleic acid,a fluorimeter for detecting fluorescence during the amplification of the nucleic acid, the fluorescence obtained from a fluorescent entity capable of providing a signal related to the quantity of the nucleic acid, anda processor for performing analysis routines, wherein the processor is programmed i) to initiate analysis algorithms by inputting into the algorithms fluorescence values measured by the fluorimeter prior to completion of temperature cycling, ii) to initiate a first algorithm to obtain a first score from a first test by inputting into the first algorithm the fluorescence values, iii) to initiate a second algorithm to obtain a second score from a second test by inputting into the second algorithm the fluorescence values, iv) to generate a composite score by evaluating a function that depends on the first score and the second score as independent variables of the function to determine whether the sample is positive or negative for the presence of the nucleic acid, and v) to evaluate the function that depends on the first score and the second score as independent variables of the function to determine whether the sample is indeterminate for the presence of the nucleic acid.
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Accused Products
Abstract
A method and device are described for analyzing a sample for the presence of a nucleic acid wherein the sample is amplified, illustratively using PCR, in the presence of a fluorescent probe capable of providing a signal related to the quantity of nucleic acid present. A nucleic acid sample is amplified in the presence of the fluorescent entity, and the fluorescence intensity is measured at each of a plurality of amplification cycles. Scores are obtained from various tests performed on the fluorescence data, and the scores are used to determine whether the nucleic acid is present in the sample.
56 Citations
7 Claims
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1. A device for determining the presence of a nucleic acid in a sample comprising
an instrument for temperature cycling for analysis of real-time amplification of the nucleic acid, a fluorimeter for detecting fluorescence during the amplification of the nucleic acid, the fluorescence obtained from a fluorescent entity capable of providing a signal related to the quantity of the nucleic acid, and a processor for performing analysis routines, wherein the processor is programmed i) to initiate analysis algorithms by inputting into the algorithms fluorescence values measured by the fluorimeter prior to completion of temperature cycling, ii) to initiate a first algorithm to obtain a first score from a first test by inputting into the first algorithm the fluorescence values, iii) to initiate a second algorithm to obtain a second score from a second test by inputting into the second algorithm the fluorescence values, iv) to generate a composite score by evaluating a function that depends on the first score and the second score as independent variables of the function to determine whether the sample is positive or negative for the presence of the nucleic acid, and v) to evaluate the function that depends on the first score and the second score as independent variables of the function to determine whether the sample is indeterminate for the presence of the nucleic acid.
Specification
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- Resources
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Current AssigneeBioFire Diagnostics Incorporated (Compagnie Merieux Alliance SAS)
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Original AssigneeIdaho Technology
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InventorsEyre, David J.
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Primary Examiner(s)Smith; Carolyn L.
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Application NumberUS10/074,178Publication NumberTime in Patent Office2,282 DaysField of Search702/19US Class Current702/19CPC Class CodesB01L 7/52 with provision for submitti...C12Q 1/6851 Quantitative amplificationC12Q 2537/165 Mathematical modelling, e.g...C12Q 2561/101 Taqman
