Electroprocessed material made by simultaneously electroprocessing a natural protein polymer and two synthetic polymers

• US 7,374,774 B2
• Filed: 09/22/2003
• Issued: 05/20/2008
• Est. Priority Date: 08/31/1999
• Status: Expired due to Fees

• Current Assignee: Virginia Commonwealth University
• Original Assignee: Virginia Commonwealth University
• Inventors: Simpson, David G., Bowlin, Gary L., Wnek, Gary E.
• Primary Examiner(s): Prouty; Rebecca E.
• Assistant Examiner(s): Kosson; Rosanne
• Application Number:
  US10/668,085
• Publication Number:
  US 20040058887A1
• Time in Patent Office: 1,702 Days
• Field of Search: 424/422, 424/423, 424/443, 424/485, 424/486, 530/353, 530/356, 428/311.11, 514/12, 514/801
• US Class Current: 424/423
• CPC Class Codes: A61F 2/08 Muscles; Tendons; Ligaments sutures A61B17/04
• CPC Class Codes: A61K 2035/126 Immunoprotecting barriers, e.g. jackets, diffusion chambers
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61L 15/32 Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin A61L15/225 takes precedence
• CPC Class Codes: A61L 2400/04 Materials for stopping bleeding
• CPC Class Codes: A61L 2430/30 for muscle reconstruction
• CPC Class Codes: A61L 27/18 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 27/225 Fibrin; Fibrinogen
• CPC Class Codes: A61L 27/24 Collagen
• CPC Class Codes: A61L 27/26 Mixtures of macromolecular compounds
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61L 27/3847 Bones
• CPC Class Codes: A61L 27/507 for artificial blood vessels apparatus for applying cells on a blood vessel prosthesis A61F2/062
• CPC Class Codes: A61L 31/041 Mixtures of macromolecular compounds
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 19/08 for bone diseases, e.g. rachitism, Paget's disease
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 31/00 Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: B29C 41/006 using an electrostatic field for applying the material
• CPC Class Codes: B29C 67/0007 : Manufacturing coloured articles not otherwise provided for, e.g. by colour change
• CPC Class Codes: C07K 14/75 : Fibrinogen
• CPC Class Codes: C07K 14/78 : Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
• CPC Class Codes: C08L 67/04 : Polyesters derived from hydroxycarboxylic acids, e.g. lactones C08L67/06 takes precedence
• CPC Class Codes: C08L 89/00 : Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00
• CPC Class Codes: C08L 89/06 : derived from leather or skin , e.g. gelatin
• CPC Class Codes: C12N 2503/00 : Use of cells in diagnostics
• CPC Class Codes: C12N 2510/00 : Genetically modified cells
• CPC Class Codes: C12N 2533/40 : Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
• CPC Class Codes: C12N 2533/54 : Collagen; Gelatin
• CPC Class Codes: C12N 2533/56 : Fibrin; Thrombin
• CPC Class Codes: C12N 5/0012 : Cell encapsulation
• CPC Class Codes: C12N 5/0062 : General methods for three-dimensional culture
• CPC Class Codes: C12N 5/0068 : General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0622 : Glial cells, e.g. astrocytes, oligodendrocytes; Schwann cells

Abstract

The invention is directed to novel compositions comprising an electroprocessed material and a substance, their formation and use. The electroprocessed material can, for example, be one or more natural materials, one or more synthetic materials, or a combination thereof. The substance can be one or more therapeutic or cosmetic substances or other compounds, molecules, cells, vesicles. The compositions can be used in substance delivery, including drug delivery within an organism by, for example, releasing substances or containing cells that release substances. The compositions can be used for other purposes, such as prostheses or similar implants.

Claims

1. A composition comprising:

an electroprocessed material and a substance, wherein the electroprocessed material is made by a method comprising the step of simultaneously electroprocessing a natural polymer that is an extracellular matrix protein or polymerized thrombin and two or more synthetic polymers, wherein the synthetic polymers are selected from the group consisting of poly(urethanes), poly(siloxanes), silicones, poly(ethylene), poly(vinyl pyrrolidone), poly(2-hydroxyethyl methacrylate), poly(N-vinyl pyrrolidone), poly(methyl methacrylate), poly(vinyl alcohol), poly(acrylic acid), polyacrylamide, poly(ethylene-co-vinyl acetate), poly(ethylene glycol), poly(methacrylic acid), polyactides (PLA), polyglycolides (PGA), poly(lactide-co-glycolides) (PLGA), polyanhydrides and polyorthoesters, and, wherein the substance comprises a therapeutic substance, a cosmetic substance or a combination thereof.

2. The composition of claim 1, wherein the therapeutic substance is selected from the group consisting of anesthetics, hypnotics, sedatives, sleep inducers, antipsychotics, antidepressants, antiallergics, antianginals, antiarthritics, antiasthmatics, antidiabetics, antidiarrheal drugs, anticonvulsants, antigout drugs, antihistamines, antipruritics, emetics, antiemetics, antispasmondics, appetite suppressants, neuroactive substances, neurotransmitter agonists, antagonists, receptor blockers, reuptake modulators, beta-adrenergic blockers, calcium channel blockers, disulfarim, muscle relaxants, analgesics, antipyretics, stimulants, anticholinesterase agents, parasympathomimetic agents, hormones, anticoagulants, antithrombotics, thrombolytics, immunoglobulins, immunosuppressants, hormone agonists, hormone antagonists, vitamins, antimicrobial agents, antineoplastics, antacids, digestants, laxatives, cathartics, antiseptics, diuretics, disinfectants, fungicides, ectoparasiticides, antiparasitics, heavy metals, heavy metal antagonists, chelating agents, alkaloids, salts, ions, autacoids, digitalis, cardiac glycosides, antiarrhythmics, antihypertensives, vasodilators, vasoconstrictors, antimuscarinics, ganglionic stimulating agents, ganglionic blocking agents, neuromuscular blocking agents, adrenergic nerve inhibitors, anti-oxidants, anti-inflammatories, wound care products, antithrombogenic agents, antitumoral agents, antithrombogenic agents, antiangiogenic agents, antigenic agents, wound healing agents, plant extracts, growth factors, growth hormones, cytokines, immunoglobulins, osteoblasts, myoblasts, neuroblasts, fibroblasts, glioblasts;

germ cells, hepatocytes, chondrocytes, keratinocytes, smooth muscle cells, cardiac muscle cells, connective tissue cells, epithelial cells, endothelial cells, hormone-secreting cells, neurons, emollients, humectants, anti-rejection drugs, spermicides, conditioners, antibacterial agents, antifungal agents, antiviral agents, antibiotics, tranquilizers, cholesterol-reducing drugs, antitussives, histamine-blocking drugs and monoamine oxidase inhibitor.

3. The composition of claim 1, wherein the electroprocessed material is crosslinked.

14. The composition of claim 1, wherein the substance is selected from the group consisting of hormones, growth factors, growth hormones, cytokines, immunoglobulins, osteoblasts, keratinocytes, cardiac muscle cells, hormone-secreting cells, antibacterial agents and antibiotics.

15. The composition of claim 1, wherein the extracellular matrix protein comprises collagen.

18. The composition of claim 1, wherein the extracellular matrix protein is selected from the group consisting of collagen, fibrin, fibrinogen, elastin, laminin and fibronectin.

19. The composition of claim 1, further comprising one or more molecules, cells, objects or combinations thereof.

4. A composition comprising an electroprocessed material, wherein the electroprocessed material is made by a process comprising the step of simultaneously electroprocessing a solution comprising a natural polymer that is an extracellular matrix protein or polymerized thrombin and two or more synthetic polymers, wherein the synthetic polymers are selected from the group consisting of poly(urethanes), poly(siloxanes), silicones, poly(ethylene), poly(vinyl pyrrolidone), poly(2-hydroxy ethyl methacrylate), poly(N-vinyl pyrrolidone), poly(methyl methacrylate), poly(vinyl alcohol), poly(acrylic acid), polyacrylamide, poly(ethylene-co-vinyl acetate), poly(ethylene glycol), poly(methacrylic acid), polylactides (PLA), polyplycolides (PGA), poly(lactide-co-glycolides) (PLGA), polyanhydrides and polyorthoesters.

5. The composition of claim 4, wherein the extracellular matrix protein is selected from the group consisting of collagen, fibrin, fibrinogen, elastin, laminin and fibronectin.

6. The composition of claim 5, wherein the electroprocessed material comprises collagen and two or more polymers, and wherein the electroprocessed collagen comprises a repeated longitudinal banding pattern, wherein the repeated longitudinal banding pattern occurs at a spacing of about 65 nm to 67 nm.

7. The composition of claim 5, wherein the electroprocessed material comprises one or more collagen types and two or more polymers.

8. The composition of claim 4, further comprising one or more molecules, cells, objects or combinations thereof.

9. The composition of claim 4, further comprising a therapeutic substance, a cosmetic substance or a combination thereof.

10. The composition of claim 9, wherein the therapeutic substance is selected from the group consisting of anesthetics, hypnotics, sedatives, sleep inducers, antipsychotics, antidepressants, antiallergics, antianginals, antiarthritics, antiasthmatics, antidiabetics, antidiarrheal drugs, anticonvulsants, antigout drugs, antihistamines, antipruritics, emetics, antiemetics, antispasmondics, appetite suppressants, neuroactive substances, neurotransmitter agonists, antagonists, receptor blockers, reuptake modulators, beta-adrenergic blockers, calcium channel blockers, disulfarim, muscle relaxants, analgesics, antipyretics, stimulants, anticholinesterase agents, parasympathomimetic agents, hormones, anticoagulants, antithrombotics, thrombolytics, immunoglobulins, immunosuppressants, hormone agonists, hormone antagonists, vitamins, antimicrobial agents, antineoplastics, antacids, digestants, laxatives, cathartics, antiseptics, diuretics, disinfectants, fungicides, ectoparasiticides, antiparasitics, heavy metals, heavy metal antagonists, chelating agents, alkaloids, salts, ions, autacoids, digitalis, cardiac glycosides, antiarrhythmics, antihypertensives, vasodilators, vasoconstrictors, antimuscarinics, ganglionic stimulating agents, ganglionic blocking agents, neuromuscular blocking agents, adrenergic nerve inhibitors, anti-oxidants, anti-inflammatories, wound care products, antithrombogenic agents, antitumoral agents, antithrombogenic agents, antiangiogenic agents, antigenic agents, wound healing agents, plant extracts, growth factors, growth hormones, cytokines, immunoglobulins, osteoblasts, myoblasts, neuroblasts, fibroblasts, glioblasts;

germ cells, hepatocytes, chondrocytes, keratinocytes, smooth muscle cells, cardiac muscle cells, connective tissue cells, epithelial cells, endothelial cells, hormone-secreting cells, neurons, emollients, humectants, anti-rejection drugs, spermicides, conditioners, antibacterial agents, antifungal agents, antiviral agents, antibiotics, tranquilizers, cholesterol-reducing drugs, antitussives, histamine-blocking drugs and monoamine oxidase inhibitor.

16. The composition of claim 4, wherein the extracellular matrix protein comprises collagen.

11. A composition comprising:

an electroprocessed material, wherein the electroprocessed material is made by a process comprising the step of simultaneously electroprocessing from a solution a natural polymer that is an extracellular matrix protein or polymerized thrombin and two or more synthetic polymers, wherein the synthetic polymers are selected from the group consisting of poly(urethanes), poly(siloxanes), silicones, poly(ethylene), poly(vinyl pyrrolidone), poly(2-hydroxy ethyl methacrylate), poly(N-vinyl pyrrolidone), poly(methyl methacrylate), poly(vinyl alcohol), poly(acrylic acid), polyacrylamide, poly(ethylene-co-vinyl acetate), poly(ethylene glycol), poly(methacrylic acid), polylactides (PLA), polyglycolides (PGA), poly(lactide-co-glycolides) (PLGA), polyanhydrides and polyorthoesters, and wherein the electroprocessing is selected from the group consisting of electrospraying, electrosputtering, electrospinning or electroaerosol treatment of the solution.

12. The composition of claim 11, further comprising a substance, wherein the substance comprises a therapeutic substance, a cosmetic substance or a combination thereof.

13. The composition of claim 12, wherein the substance is selected from the group consisting of hormones, growth factors, growth hormones, cytokines, immunoglobulins, osteoblasts, keratinocytes, cardiac muscle cells, hormone-secreting cells, antibacterial agents and antibiotics.

17. The composition of claim 11, wherein the extracellular matrix protein comprises collagen.