GLP-1 gene delivery for the treatment of type 2 diabetes

• US 7,374,930 B2
• Filed: 05/21/2002
• Issued: 05/20/2008
• Est. Priority Date: 05/21/2002
• Status: Active Grant

• Current Assignee: CLSN Laboratories, Inc. (Imunon, Inc.)
• Original Assignee: EGEN, Inc. (Imunon, Inc.)
• Inventors: Lee, Minhyung, Ko, Kyungsoo, Oh, Seungjoon
• Primary Examiner(s): QIAN, CELINE X
• Application Number:
  US10/153,470
• Publication Number:
  US 20030220274A1
• Time in Patent Office: 2,191 Days
• Field of Search: None
• US Class Current: 435/320.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 47/59 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
• CPC Class Codes: A61K 47/593 Polyesters, e.g. PLGA or polylactide-co-glycolide
• CPC Class Codes: A61K 47/645 Polycationic or polyanionic oligopeptides, polypeptides or polyamino acids, e.g. polylysine, polyarginine, polyglutamic acid or peptide TAT
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: C07K 14/605 Glucagons

Abstract

This patent discloses compositions and methods of use thereof to normalize the blood glucose levels of patients with type 2 diabetes. It relates particularly to a plasmid comprising a chicken β actin promoter and enhancer; a modified GLP-1 (7-37) cDNA (pβGLP1), carrying a furin cleavage site, which is constructed and delivered into a cell for the expression of active GLP-1.

Claims

1. A plasmid comprising the nucleotide sequence of SEQ ID NO:

1.