In vivo synthesis of connective tissues
First Claim
1. A biocompatible scaffold comprising multiple layers and seeded with precursor cells, wherein the biocompatible scaffold is comprised of a gelatin layer that is sandwiched between two collagen sponge layers or one collagen layer sandwiched between two gelatin layers.
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Abstract
The in vivo synthesis of connective tissue by fibroblast or fibroblast precursor cells ensconced within a biocompatible scaffold is disclosed. The cells are preferably present in a biocompatible scaffold such as gelatin and placed between two other biocompatible scaffolds such as collagen sponges soaked with a collagenic amount of a member of the TGF-β family of proteins. This composition is then implanted in a host to produce cranial sutures, periodontal ligament or other fibrous tissue structures in vivo.
49 Citations
20 Claims
- 1. A biocompatible scaffold comprising multiple layers and seeded with precursor cells, wherein the biocompatible scaffold is comprised of a gelatin layer that is sandwiched between two collagen sponge layers or one collagen layer sandwiched between two gelatin layers.
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11. A composition that comprises precursor cells that are seeded in a biocompatible scaffold comprised of a gelatin layer sandwiched between two collagen sponge layers, wherein one or both collagen sponge layers contains a growth factor protein that is a member of the TGF-β
- family of proteins, wherein the growth factor protein is present in an amount effective to activate proliferation and/or differentiation of the precursor cells.
- View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19)
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20. A composition that comprises fibroblastic cells that are seeded in a biocompatible scaffold comprised of a gelatin layer that is sandwiched between two collagen sponge layers, wherein one or both collagen sponge layers contains a member of the TGF-β
- family of proteins in an amount effective to promote production of extracellular matrix by the cells.
Specification