Micro UV detector

• US 7,375,348 B1
• Filed: 11/03/2005
• Issued: 05/20/2008
• Est. Priority Date: 03/07/2005
• Status: Active Grant

• Current Assignee: The United States of America As Represented By The Secretary of Agriculture
• Original Assignee: The United States Of America As Represented By The Secretary Of The Army
• Inventors: Sickenberger, Richard, Cabalo, Jerry B., Sickenberger, David W.
• Primary Examiner(s): Hannaher; Constantine
• Assistant Examiner(s): Green; Yara B
• Application Number:
  US11/268,758
• Time in Patent Office: 929 Days
• Field of Search: None
• US Class Current: 250/461.2
• CPC Class Codes: G01N 15/06 Investigating concentration of particle suspensions by weighing G01N5/00; investigating sedimentation of particle suspensions G01N15/04; investigating individual particles G01N15/10
• CPC Class Codes: G01N 15/0656 using electric, e.g. electrostatic methods or magnetic methods by investigating individual particles G01N15/1031, G01N15/12
• CPC Class Codes: G01N 15/075 by optical means
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence
• CPC Class Codes: G01N 2201/065 Integrating spheres

Abstract

A biological aerosol detector is provided. The biological aerosol detector uses a semiconductor optical source with an ultraviolet emission band to excite biological molecules in an aerosol sample. Filtering optics are configured to attenuate radiation from a secondary emission band of the optical source to prevent false signals due to scattering of secondary emission band radiation from non-biological molecules. An intake/exhaust manifold that includes an intake pipe that fits within a concentric exhaust pipe is also provided. The intake/exhaust manifold planarizes the flow of the sampled aerosol to maximize the time of irradiation. An electrostatic sampling grid is also provided to selectively draw biological molecules having a net charge into the optical chamber.

Claims

1. A biological aerosol detector comprising:

an excitation source having a primary emission band and a secondary emission band, wherein the primary emission band is configured to excite a sample;

an optical cavity in which the sample is excited by radiation from the excitation source;

filtering optics configured to attenuate radiation emitted in the secondary emission band; and

a housing for an elliptical mirror that creates the optical cavity and an orifice in the housing that acts as a control aperture to limit stray radiation from entering the optical cavity, and wherein the orifice comprises a UV absorptive material.

2. The biological aerosol detector of claim 1, wherein the excitation source is a semiconductor ultraviolet optical source and the primary emission band is within the ultraviolet spectrum.

3. The biological aerosol detector of claim 2, wherein the secondary emission band corresponds to longer wavelength emissions that overlap the emissions from a sample excited by the excitation source'"'"'s primary emission band.

4. The biological aerosol detector of claim 1, wherein the filtering optics comprise a dichroic mirror configured to reflect radiation in one of the primary or secondary emission bands and to transmit radiation in the other of the primary or secondary emission bands.

5. The biological aerosol detector of claim 4, further comprising a focusing optic between the excitation source and the dichroic mirror.

6. The biological aerosol detector of claim 5, wherein the focusing optic is a ball lens.

7. The biological aerosol detector of claim 4, wherein the filtering optics further comprise an optical filter configured to attenuate the intensity of radiation in the secondary emission band.

8. The biological aerosol detector of claim 7, wherein the optical filter is positioned in the optical path between the dichroic mirror and the optical cavity.

9. The biological aerosol detector of claim 7, wherein the filtering optics further comprise a lens positioned in the optical path between the dichroic mirror and the optical filter.

10. The biological aerosol detector of claim 1, wherein the UV absorptive material is graphite.

11. The biological aerosol detector of claim 1, wherein the orifice is located at least partially within the optical cavity.

12. A biological aerosol detector comprising:

an optical excitation source;

an optical cavity in which a sampled aerosol is irradiated by the optical excitation source;

a housing, enclosing the optical cavity; and

a combined intake and exhaust gas manifold passing through the housing and into the optical cavity, wherein the combined intake and exhaust gas manifold collects the sampled aerosol and directs it into the optical cavity, and exhausts the sampled aerosol from the optical cavity, and wherein the combined intake and exhaust gas manifold samples the aerosol and directs it into the optical cavity and exhausts the sampled aerosol from the optical cavity in the same plane, and further wherein the combined intake and exhaust gas manifold further comprises;

an intake pipe of a first length having one end external to and one end internal to the housing forming the optical cavity;

an exhaust pipe of a second length that is less than the first length, having one end external to and one end internal to the housing forming the optical cavity the exhaust pipe completely surrounding the intake pipe over a portion of the length of the intake pipe lying between the ends of the intake pipe.

13. The biological aerosol detector of claim 12, wherein the combined intake and exhaust gas manifold further comprises an end cap having a face plate with a hole bored therethrough, wherein the end cap covers the end of the exhaust pipe that is external to the housing, and wherein the intake pipe passes through the hole bored through the end cap'"'"'s face plate.

14. A biological aerosol detector comprising:

an optical excitation source;

an optical cavity in which a sampled aerosol is irradiated by the optical excitation source;

a housing, enclosing the optical cavity having two holes bored therethrough along a line that forms a sampling axis;

a pair of electrical grids located external to the housing and on either side of the holes that form the sampling axis; and

a power source attached to the pair of electrical grids to generate an electric field between the pair of electrical grids, the electric field causing the preferential sampling of aerosol molecules having a net electrical charge.

15. The biological aerosol detector of claim 14, wherein the electrical grids are plates or meshes.

16. The biological aerosol detector of claim 14, further comprising a Faraday based ion detector to detect sampled aerosol molecules having a net electrical change.

17. The biological aerosol detector of claim 16, further comprising:

an optical detection circuit to detect a fluorescence signal emitted by a sampled aerosol molecule that has been excited by the optical excitation source; and

a correlation circuit to correlate signals from the optical detection circuit and the Faraday based ion detector.

18. The biological aerosol detector of claim 17, wherein the correlation circuit is a temporal coincidence circuit.

19. The biological aerosol detector of claim 16, wherein the Faraday based ion detector comprises a collection grid and an ion detection circuit.