Methods for chemically synthesizing immunoglobulin chimeric proteins
First Claim
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1. A method of producing a chimeric protein comprising combining(a) at least a portion of an immunoglobulin constant region comprising an FcRn binding site and having a naturally occurring cysteine from the interchain region of the immunoglobulin as the N-terminal amino acid;
- and(b) a biologically active molecule comprising a functional group capable of reacting with an N terminal cysteine;
wherein the combining of (a) and (b) produces the chimeric protein.
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Abstract
The invention provides methods of chemically synthesizing chimeric proteins comprising at least a portion of an immunoglobulin constant region and a biologically active molecule.
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26 Claims
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1. A method of producing a chimeric protein comprising combining
(a) at least a portion of an immunoglobulin constant region comprising an FcRn binding site and having a naturally occurring cysteine from the interchain region of the immunoglobulin as the N-terminal amino acid; - and
(b) a biologically active molecule comprising a functional group capable of reacting with an N terminal cysteine; wherein the combining of (a) and (b) produces the chimeric protein. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A method of producing a monomer-dimer hybrid protein comprising combining
(a) at least a portion of an immunoglobulin constant region comprising an FcRn binding site and having a naturally occurring cysteine from the interchain region of the immunoglobulin as the N-terminal amino acid; - and
(b) a biologically active molecule comprising a functional group capable of reacting with an N terminal cysteine; wherein the combining of (a) and (b) produces the monomer-dimer hybrid protein. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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Specification