Kits for detection of nucleic acids by invasive cleavage
First Claim
1. A kit for detecting a target sequence, comprising:
- i) nucleic acid molecules capable of forming an invasive cleavage structure in the presence of a target sequence; and
ii) a thermostable Flap endonuclease.
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Abstract
The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.
76 Citations
16 Claims
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1. A kit for detecting a target sequence, comprising:
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i) nucleic acid molecules capable of forming an invasive cleavage structure in the presence of a target sequence; and ii) a thermostable Flap endonuclease. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A kit for detecting a target sequence, comprising:
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a) nucleic acid molecules configured to form an invasive cleavage structure on a target sequence that is cleavable by a 5′
-nuclease, wherein said invasive cleavage structure comprises;i) said target sequence; ii) nucleic acid molecules that form an invasive cleavage structure with a displaced 5′
nucleic acid arm in the presence of said target sequence; andb) a thermostable Flap-endonuclease. - View Dependent Claims (15, 16)
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Specification