Optical biosensor with enhanced activity retention for detection of halogenated organic compounds
First Claim
1. A distal tip of a biosensor ion sensing transducer for use in detecting an analyte comprising a halogenated organic compound, the tip comprising:
- a biocomponent comprising at least one enzyme selected from the group consisting of hydrolases, subclass EC 3.8, and lyases, subclass EC 4.5, for carrying out a dehalogenation of the compound, said biocomponent immobilized to a surface of the tip by means selected from the group consisting of (a) entrapment within a hydrogel;
(b) entrapment within a polymeric network;
(c) encapsulation;
(d) covalent-bonding; and
(e) adsorption;
wherein said biocomponent has been treated to maintain a period of enzymatic efficacy; and
wherein said biocomponent is stabilized to said tip by means selected from the group consisting of crosslinking a surface of said immobilized biocomponent, crosslinking a polymer layer to said biocomponent, adding a gel-hardening agent to said biocomponent, adding a stabilizing agent to said biocomponent, and modifying a component used to immobilize said biocomponent to said surface of the tip.
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Accused Products
Abstract
A distal tip, and associated method of producing, of a biosensor ion sensing transducer for use in detecting an analyte comprising an halogenated organic compound in an environment such as soil or an aqueous environment. Features include: a biocomponent comprising at least one enzyme for carrying out a dehalogenation of the compound; the biocomponent is immobilized to a surface of the tip; a treatment of the biocomponent for maintaining a period of enzymatic efficacy; and the biocomponent stabilized by means preferably selected from the group consisting of crosslinking a surface of the immobilized biocomponent, crosslinking a polymer layer to the biocomponent, adding a gel-hardening agent to the biocomponent, adding a stabilizing agent to the biocomponent, and modifying a component of the immobilizing means. Immobilization can be carried out by: (a) entrapment within a hydrogel; (b) entrapment within a polymeric network; (c) (micro)encapsulation; (d) covalent-bonding; and (e) adsorption. The dehalogenase of the biocomponent may be selected from the group consisting of hydrolases, subclass EC 3.8, and lyases, subclass EC 4.5 as listed in Table 2A.
27 Citations
18 Claims
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1. A distal tip of a biosensor ion sensing transducer for use in detecting an analyte comprising a halogenated organic compound, the tip comprising:
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a biocomponent comprising at least one enzyme selected from the group consisting of hydrolases, subclass EC 3.8, and lyases, subclass EC 4.5, for carrying out a dehalogenation of the compound, said biocomponent immobilized to a surface of the tip by means selected from the group consisting of (a) entrapment within a hydrogel;
(b) entrapment within a polymeric network;
(c) encapsulation;
(d) covalent-bonding; and
(e) adsorption;wherein said biocomponent has been treated to maintain a period of enzymatic efficacy; and wherein said biocomponent is stabilized to said tip by means selected from the group consisting of crosslinking a surface of said immobilized biocomponent, crosslinking a polymer layer to said biocomponent, adding a gel-hardening agent to said biocomponent, adding a stabilizing agent to said biocomponent, and modifying a component used to immobilize said biocomponent to said surface of the tip. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A distal tip of a biosensor ion sensing transducer for use in detecting an analyte comprising a halogenated organic compound, the tip comprising:
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a biocomponent comprising at least one enzyme selected from the group consisting of hydrolases, subclass EC 3.8, and lyases, subclass EC 4.5, for carrying out a dehalogenation of the compound, said biocomponent immobilized to a surface of the tip by means selected from the group consisting of (a) entrapment within a hydrogel;
(b) entrapment within a polymeric network;
(c) encapsulation;
(d) covalent-bonding; and
(e) adsorption,wherein said biocomponent is stabilized to said tip by means selected from the group consisting of crosslinking a surface of said immobilized biocomponent, crosslinking a polymer layer to said biocomponent, adding a gel-hardening agent to said biocomponent, adding a stabilizing agent to said biocomponent, and modifying a component used to immobilize said biocomponent to said surface of the tip. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18)
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Specification