Device and method for multiple analyte detection
First Claim
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1. A system comprising:
- a device comprisinga plurality of detection chambers,channel means in fluid communication with each of the plurality of detection chambers, wherein the channel means provides a plurality of dead-end fluid connections to the plurality of detection chambers, wherein one dead-end fluid connection is provided to each of the plurality of detection chambers, and each dead-end fluid connection provides the sole fluid access to its respective detection chamber such that fluid cannot enter or exit the respective detection chamber by any other way than through the respective dead-end fluid connection,non-immobilized detection reagents provided in each of the plurality of detection chambers, the non-immobilized detection reagents comprising at least one binding polymer, wherein the non-immobilized detection reagents are effective to selectively bind with a target nucleic acid sequence under conditions effective to produce a detectable fluorescent signal when a target nucleic acid sequence is present in a liquid sample in at least one of the plurality of detection chambers; and
a fluorescence detector for detecting a fluorescent signal, the fluorescence detector configured for optical communication with one or more of the plurality of detection chambers.
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Abstract
The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.
73 Citations
21 Claims
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1. A system comprising:
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a device comprising a plurality of detection chambers, channel means in fluid communication with each of the plurality of detection chambers, wherein the channel means provides a plurality of dead-end fluid connections to the plurality of detection chambers, wherein one dead-end fluid connection is provided to each of the plurality of detection chambers, and each dead-end fluid connection provides the sole fluid access to its respective detection chamber such that fluid cannot enter or exit the respective detection chamber by any other way than through the respective dead-end fluid connection, non-immobilized detection reagents provided in each of the plurality of detection chambers, the non-immobilized detection reagents comprising at least one binding polymer, wherein the non-immobilized detection reagents are effective to selectively bind with a target nucleic acid sequence under conditions effective to produce a detectable fluorescent signal when a target nucleic acid sequence is present in a liquid sample in at least one of the plurality of detection chambers; and a fluorescence detector for detecting a fluorescent signal, the fluorescence detector configured for optical communication with one or more of the plurality of detection chambers. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A device comprising:
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a plurality of detection chambers; channel means in fluid communication with each of the plurality of detection chambers, wherein the channel means provides a plurality of dead-end fluid connections to the plurality of detection chambers, wherein one dead-end fluid connection is provided to each of the plurality of detection chambers, and each dead-end fluid connection provides the sole fluid access to its respective detection chamber such that fluid cannot enter or exit the respective detection chamber by other way than through the respective dead-end fluid connection; and non-immobilized detection reagents provided in each of the plurality of detection chambers, the non-immobilized detection reagents comprising at least one binding polymer, wherein the non-immobilized detection reagents are effective to selectively bind with a target nucleic acid sequence under conditions effective to produce a detectable fluorescent signal when a target nucleic acid sequence is present in a liquid sample in at least one of the plurality of detection chambers. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16)
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17. A system for detecting or quantitating one or more target nucleic acid sequences in a liquid sample, the system comprising:
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a device comprising a plurality of detection chambers, a channel network in fluid communication with each of the plurality of detection chambers, the channel network comprising a plurality of dead-end fluid connections to the plurality of detection chambers, wherein one dead-end fluid connection is provided to each of the plurality of detection chambers, and each dead-end fluid connection provides the sole fluid access to its respective detection chamber such that fluid cannot enter or exit the respective detection chamber by any other way than through the respective dead-end fluid connection, non-immobilized detection reagents provided in at least one of the plurality of detection chambers, the non-immobilized detection reagents comprising an unlabeled oligonucleotide primer pair effective to selectively bind with a target nucleic acid sequence under conditions effective to amplify the target nucleic acid sequence by polymerase chain reaction when the target nucleic acid sequence is present in the liquid sample in the at least one of the plurality of detection chambers; a fluorescent detection reagent provided in the at least one of the plurality of detection chambers; and a fluorescence detector for detecting a fluorescent signal, the fluorescence detector configured for optical communication with one or more of the plurality of detection chambers. - View Dependent Claims (18)
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19. A system for detecting or quantitating one or more target polynucleotide sequences in a liquid sample, comprising:
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a device comprising a substrate defining a sample-distribution network including (i) a sample inlet, (ii) two or more detection chambers, (iii) a channel providing a fluid communication to said inlet, and (iv) a plurality of dead-end fluid connections, wherein said two or more detection chambers are each pre-loaded with an oligonucleotide primer pair suitable for polymerase chain reaction; a temperature-controller adapted to heat the two or more detection chambers; and a fluorescence detector configured for optical communication with one or more of the two or more detection chambers, for detecting fluorescence produced therein. - View Dependent Claims (20, 21)
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Specification