Electrodes linked via conductive oligomers to nucleic acids
First Claim
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1. A method of detecting a target nucleic acid sequence in a nucleic acid sample comprisinga) providing a solid support comprising an array of electrodes each comprising:
- i) a covalently attached anchor sequence; and
ii) a self-assembled monolayer;
b) contacting said array with at least one target sequence and a second nucleic acid sequence comprising a first region substantially complementary to said anchor sequence and a second region substantially complementary to a region of said target sequence, to form a hybridization complex;
wherein said hybridization complex further comprises an electron transfer moiety (ETM); and
c) detecting the presence of said ETM as an indication of the presence of said target nucleic acid sequence.
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Abstract
The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids with electron transfer moieties and electrodes to produce a new class of biomaterials, and to methods of making and using them.
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12 Claims
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1. A method of detecting a target nucleic acid sequence in a nucleic acid sample comprising
a) providing a solid support comprising an array of electrodes each comprising: -
i) a covalently attached anchor sequence; and ii) a self-assembled monolayer; b) contacting said array with at least one target sequence and a second nucleic acid sequence comprising a first region substantially complementary to said anchor sequence and a second region substantially complementary to a region of said target sequence, to form a hybridization complex;
wherein said hybridization complex further comprises an electron transfer moiety (ETM); andc) detecting the presence of said ETM as an indication of the presence of said target nucleic acid sequence. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification