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Assays

  • US 7,384,796 B2
  • Filed: 12/23/2002
  • Issued: 06/10/2008
  • Est. Priority Date: 02/17/1989
  • Status: Expired due to Fees
First Claim
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1. An immunoassay method performed using an immunoassay test strip, the method comprising:

  • contacting a liquid sample with a porous receiving member of the test strip, the liquid sample comprising an analyte,flowing the liquid sample along the porous receiving member and thereafter into a macroporous body of the test strip, the macroporous body comprising, in a dried state, a labeled binding reagent comprising a colored particle and a binding portion capable of binding the analyte,flowing the liquid applied to the macroporous body therealong,mobilizing, with the liquid flowing along the macroporous body, the labeled binding reagent from the dried state within the macroporous body,flowing the liquid that comprises the mobilized labeled binding reagent into a location of a porous strip of the test strip, the porous strip comprising a detection zone,flowing the liquid applied to the porous strip along the porous strip to the detection zone of the porous strip, andimmobilizing, in the detection zone, a colored complex, the colored complex comprising the labeled binding reagent, the analyte, and a first binding reagent immobilized in the detection zone, the labeled binding reagent and the first binding reagent bound to the analyte.

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