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Human immunosuppressive protein

  • US 7,388,076 B2
  • Filed: 07/16/2003
  • Issued: 06/17/2008
  • Est. Priority Date: 07/16/2002
  • Status: Expired due to Fees
First Claim
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1. A method for purifying a human immunosuppressant protein (HISP) the method comprising:

  • a) obtaining supernatant from hNT cells;

    b) exposing the supernatant to preparative polyactylamide gel electrophoresis to produce 20 isoelectric fractions, including active isoelectric fraction #10;

    c) placing the active isoelectric fraction on a Blue Sepharose column to bind albumin; and

    d) collecting the free fraction containing the concentrated, isolated HISP.

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